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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17283 | 3' | -54.5 | NC_004367.1 | + | 42029 | 0.66 | 0.933431 |
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Target: 5'- --gGAGCCCAguUgGGAGg---CCCAg -3' miRNA: 3'- guaCUCGGGUguAgCCUCacaaGGGU- -5' |
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| 17283 | 3' | -54.5 | NC_004367.1 | + | 65430 | 0.67 | 0.904847 |
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Target: 5'- ---aAGCCCcCGUCgaaaaaGGGGUGUUCCUAg -3' miRNA: 3'- guacUCGGGuGUAG------CCUCACAAGGGU- -5' |
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| 17283 | 3' | -54.5 | NC_004367.1 | + | 122855 | 0.72 | 0.655506 |
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Target: 5'- gGUGAGCCCAg--CGGAuGUGguccUCCCAg -3' miRNA: 3'- gUACUCGGGUguaGCCU-CACa---AGGGU- -5' |
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| 17283 | 3' | -54.5 | NC_004367.1 | + | 125764 | 0.72 | 0.655506 |
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Target: 5'- gGUGAGCCCAg--CGGAuGUGguccUCCCAg -3' miRNA: 3'- gUACUCGGGUguaGCCU-CACa---AGGGU- -5' |
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| 17283 | 3' | -54.5 | NC_004367.1 | + | 128674 | 0.72 | 0.655506 |
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Target: 5'- gGUGAGCCCAg--CGGAuGUGguccUCCCAg -3' miRNA: 3'- gUACUCGGGUguaGCCU-CACa---AGGGU- -5' |
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| 17283 | 3' | -54.5 | NC_004367.1 | + | 76726 | 0.93 | 0.039299 |
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Target: 5'- aCAUGAGCCUACAUCcGAGUGUUCCCAa -3' miRNA: 3'- -GUACUCGGGUGUAGcCUCACAAGGGU- -5' |
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| 17283 | 3' | -54.5 | NC_004367.1 | + | 76762 | 1.09 | 0.003617 |
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Target: 5'- aCAUGAGCCCACAUCGGAGUGUUCCCAa -3' miRNA: 3'- -GUACUCGGGUGUAGCCUCACAAGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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