miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
17283 5' -52.5 NC_004367.1 + 109387 0.66 0.972249
Target:  5'- uCAUGAGU---CAUCCGuG-GUUCCCGa -3'
miRNA:   3'- -GUACUCGgauGUAGGCuCaCAAGGGU- -5'
17283 5' -52.5 NC_004367.1 + 43044 0.68 0.926784
Target:  5'- --aGAGCCagg--CCGAGUG-UCCCAg -3'
miRNA:   3'- guaCUCGGauguaGGCUCACaAGGGU- -5'
17283 5' -52.5 NC_004367.1 + 76798 0.69 0.896166
Target:  5'- aCAUcAGaCC-ACAUCCGAGUuUUCCCAa -3'
miRNA:   3'- -GUAcUC-GGaUGUAGGCUCAcAAGGGU- -5'
17283 5' -52.5 NC_004367.1 + 43519 0.69 0.909167
Target:  5'- --gGGGCCgaagagCCGAGUGUcCCCAg -3'
miRNA:   3'- guaCUCGGaugua-GGCUCACAaGGGU- -5'
17283 5' -52.5 NC_004367.1 + 52976 0.73 0.700266
Target:  5'- -uUGGGCUUACAUCUGAGUGaaaaagUCCaCAa -3'
miRNA:   3'- guACUCGGAUGUAGGCUCACa-----AGG-GU- -5'
17283 5' -52.5 NC_004367.1 + 76762 0.93 0.059143
Target:  5'- aCAUGAGCCcACAUCgGAGUGUUCCCAa -3'
miRNA:   3'- -GUACUCGGaUGUAGgCUCACAAGGGU- -5'
17283 5' -52.5 NC_004367.1 + 76726 1.09 0.005306
Target:  5'- aCAUGAGCCUACAUCCGAGUGUUCCCAa -3'
miRNA:   3'- -GUACUCGGAUGUAGGCUCACAAGGGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.