Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17285 | 5' | -51.9 | NC_004367.1 | + | 27282 | 0.66 | 0.986269 |
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Target: 5'- -aAUCcuCCGCUcGUCUggUACAGGCCc -3' miRNA: 3'- gaUAGucGGCGGaCAGAa-GUGUUCGG- -5' |
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| 17285 | 5' | -51.9 | NC_004367.1 | + | 57956 | 0.66 | 0.978106 |
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Target: 5'- gUGUCGGCCaCCUGgauaCUgCACAccaaauccAGCCa -3' miRNA: 3'- gAUAGUCGGcGGACa---GAaGUGU--------UCGG- -5' |
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| 17285 | 5' | -51.9 | NC_004367.1 | + | 97690 | 0.66 | 0.978106 |
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Target: 5'- gCUG-CAGCCGCuCUGUgUUCAUGucCCa -3' miRNA: 3'- -GAUaGUCGGCG-GACAgAAGUGUucGG- -5' |
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| 17285 | 5' | -51.9 | NC_004367.1 | + | 123141 | 0.67 | 0.963798 |
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Target: 5'- gUAUCugAGCCGCCaUGUUgcgagcugcugaaagUCACAguuGGCCu -3' miRNA: 3'- gAUAG--UCGGCGG-ACAGa--------------AGUGU---UCGG- -5' |
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| 17285 | 5' | -51.9 | NC_004367.1 | + | 126050 | 0.67 | 0.963798 |
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Target: 5'- gUAUCugAGCCGCCaUGUUgcgagcugcugaaagUCACAguuGGCCu -3' miRNA: 3'- gAUAG--UCGGCGG-ACAGa--------------AGUGU---UCGG- -5' |
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| 17285 | 5' | -51.9 | NC_004367.1 | + | 83636 | 0.68 | 0.947612 |
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Target: 5'- aCUAUCuccAGCCuGCUUGUCUagGaGAGCCu -3' miRNA: 3'- -GAUAG---UCGG-CGGACAGAagUgUUCGG- -5' |
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| 17285 | 5' | -51.9 | NC_004367.1 | + | 101916 | 0.68 | 0.947612 |
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Target: 5'- gUGUCAGCCGUCaaUCUUCuccCAAGUg -3' miRNA: 3'- gAUAGUCGGCGGacAGAAGu--GUUCGg -5' |
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| 17285 | 5' | -51.9 | NC_004367.1 | + | 73336 | 0.7 | 0.875996 |
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Target: 5'- ----aAGCCacGCCUGUC-UCAgAGGCCa -3' miRNA: 3'- gauagUCGG--CGGACAGaAGUgUUCGG- -5' |
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| 17285 | 5' | -51.9 | NC_004367.1 | + | 81782 | 1.13 | 0.003389 |
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Target: 5'- gCUAUCAGCCGCCUGUCUUCACAAGCCa -3' miRNA: 3'- -GAUAGUCGGCGGACAGAAGUGUUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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