Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 17286 | 3' | -56.8 | NC_004367.1 | + | 146721 | 0.7 | 0.587736 |
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Target: 5'- -cCCCUucACUGGGUCCAGucacGCuCGGGCGc -3' miRNA: 3'- uaGGGA--UGACUCAGGUC----UG-GUCCGUu -5' |
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| 17286 | 3' | -56.8 | NC_004367.1 | + | 147049 | 0.7 | 0.587736 |
|
Target: 5'- -cCCCUucACUGGGUCCAGucacGCuCGGGCGc -3' miRNA: 3'- uaGGGA--UGACUCAGGUC----UG-GUCCGUu -5' |
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| 17286 | 3' | -56.8 | NC_004367.1 | + | 147377 | 0.7 | 0.587736 |
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Target: 5'- -cCCCUucACUGGGUCCAGucacGCuCGGGCGc -3' miRNA: 3'- uaGGGA--UGACUCAGGUC----UG-GUCCGUu -5' |
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| 17286 | 3' | -56.8 | NC_004367.1 | + | 147704 | 0.7 | 0.598142 |
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Target: 5'- -gCCCUucACUGGGUCCAGucacGCuCGGGCGc -3' miRNA: 3'- uaGGGA--UGACUCAGGUC----UG-GUCCGUu -5' |
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| 17286 | 3' | -56.8 | NC_004367.1 | + | 148032 | 0.7 | 0.587736 |
|
Target: 5'- -cCCCUucACUGGGUCCAGucacGCuCGGGCGc -3' miRNA: 3'- uaGGGA--UGACUCAGGUC----UG-GUCCGUu -5' |
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| 17286 | 3' | -56.8 | NC_004367.1 | + | 148360 | 0.7 | 0.587736 |
|
Target: 5'- -cCCCUucACUGGGUCCAGucacGCuCGGGCGc -3' miRNA: 3'- uaGGGA--UGACUCAGGUC----UG-GUCCGUu -5' |
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| 17286 | 3' | -56.8 | NC_004367.1 | + | 149015 | 0.7 | 0.587736 |
|
Target: 5'- -cCCCUucACUGGGUCCAGucacGCuCGGGCGc -3' miRNA: 3'- uaGGGA--UGACUCAGGUC----UG-GUCCGUu -5' |
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| 17286 | 3' | -56.8 | NC_004367.1 | + | 149343 | 0.7 | 0.587736 |
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Target: 5'- -cCCCUucACUGGGUCCAGucacGCuCGGGCGc -3' miRNA: 3'- uaGGGA--UGACUCAGGUC----UG-GUCCGUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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