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miRNA: 3'- -ACUGgaCACAGUA-AGCCGACCAGuuu -5'
miRNA: 3'- aCugG-ACACAGUAaGCCGaCCAGUuu -5'
miRNA: 3'- -ACUGGACACAGUAAGCCgAcCAgUUU- -5'
miRNA: 3'- -ACUGGACACAGUAAGCCGACCAGUUU- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 17288 | 3' | -51.9 | NC_004367.1 | + | 74411 | 0.68 | 0.945781 |
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Target: 5'- cUGACaaGUGUUGUgUUGGCUGGUCc-- -3' miRNA: 3'- -ACUGgaCACAGUA-AGCCGACCAGuuu -5' |
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| 17288 | 3' | -51.9 | NC_004367.1 | + | 64280 | 0.71 | 0.821968 |
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Target: 5'- aGugCaUGUGUCAUgCGGCaGGUCAu- -3' miRNA: 3'- aCugG-ACACAGUAaGCCGaCCAGUuu -5' |
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| 17288 | 3' | -51.9 | NC_004367.1 | + | 88427 | 0.77 | 0.523742 |
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Target: 5'- aUGACCUGUGUCAUUCGGgU-GUgAAAa -3' miRNA: 3'- -ACUGGACACAGUAAGCCgAcCAgUUU- -5' |
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| 17288 | 3' | -51.9 | NC_004367.1 | + | 88564 | 1.07 | 0.007645 |
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Target: 5'- aUGACCUGUGUCAUUCGGCUGGUCAAAa -3' miRNA: 3'- -ACUGGACACAGUAAGCCGACCAGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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