Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17290 | 5' | -52.4 | NC_004367.1 | + | 56079 | 0.67 | 0.971662 |
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Target: 5'- uGGGCaaa-UGUCCGCGGgacGGGGAc -3' miRNA: 3'- cCCUGaauaACAGGCGCCagaCUCCU- -5' |
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| 17290 | 5' | -52.4 | NC_004367.1 | + | 22508 | 0.67 | 0.971662 |
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Target: 5'- uGGGCaaa-UGUCCGCGGgacGGGGAc -3' miRNA: 3'- cCCUGaauaACAGGCGCCagaCUCCU- -5' |
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| 17290 | 5' | -52.4 | NC_004367.1 | + | 118402 | 0.68 | 0.954638 |
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Target: 5'- aGGGAaugg-UG-CUGUGGUUUGAGGGu -3' miRNA: 3'- -CCCUgaauaACaGGCGCCAGACUCCU- -5' |
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| 17290 | 5' | -52.4 | NC_004367.1 | + | 115564 | 0.68 | 0.946216 |
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Target: 5'- gGGGACa-GUUGUCCGCGagacGGGGAc -3' miRNA: 3'- -CCCUGaaUAACAGGCGCcagaCUCCU- -5' |
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| 17290 | 5' | -52.4 | NC_004367.1 | + | 55828 | 0.73 | 0.772649 |
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Target: 5'- gGGGACaac-UGUCCGCGGgacGGGGAc -3' miRNA: 3'- -CCCUGaauaACAGGCGCCagaCUCCU- -5' |
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| 17290 | 5' | -52.4 | NC_004367.1 | + | 55864 | 0.75 | 0.672397 |
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Target: 5'- cGGGACggGUacaacUGUCCGCGGgc-GGGGAc -3' miRNA: 3'- -CCCUGaaUA-----ACAGGCGCCagaCUCCU- -5' |
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| 17290 | 5' | -52.4 | NC_004367.1 | + | 22298 | 0.76 | 0.589233 |
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Target: 5'- gGGGACUac-UGUCCGCGGgacGGGGAc -3' miRNA: 3'- -CCCUGAauaACAGGCGCCagaCUCCU- -5' |
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| 17290 | 5' | -52.4 | NC_004367.1 | + | 22256 | 0.76 | 0.589233 |
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Target: 5'- gGGGACUac-UGUCCGCGGgacGGGGAc -3' miRNA: 3'- -CCCUGAauaACAGGCGCCagaCUCCU- -5' |
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| 17290 | 5' | -52.4 | NC_004367.1 | + | 115522 | 0.77 | 0.538034 |
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Target: 5'- gGGGACa-GUUGUCCGCGGgacGGGGAc -3' miRNA: 3'- -CCCUGaaUAACAGGCGCCagaCUCCU- -5' |
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| 17290 | 5' | -52.4 | NC_004367.1 | + | 115575 | 1.11 | 0.004984 |
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Target: 5'- uGGGACUUAUUGUCCGCGGUCUGAGGAc -3' miRNA: 3'- -CCCUGAAUAACAGGCGCCAGACUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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