Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17298 | 3' | -57 | NC_004367.1 | + | 38145 | 0.66 | 0.855233 |
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Target: 5'- uUUCUUCACCUg-CCGUgCGUUAAGGCg -3' miRNA: 3'- uAAGGGGUGGAagGGCG-GCGAUUCUG- -5' |
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| 17298 | 3' | -57 | NC_004367.1 | + | 1143 | 0.66 | 0.847288 |
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Target: 5'- --gCCCCAC--UCCCGCCGgUGuuuaucGACa -3' miRNA: 3'- uaaGGGGUGgaAGGGCGGCgAUu-----CUG- -5' |
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| 17298 | 3' | -57 | NC_004367.1 | + | 74320 | 0.66 | 0.839145 |
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Target: 5'- -aUCCCC-CUaagUUCCCcacuGCCGC-AGGACa -3' miRNA: 3'- uaAGGGGuGG---AAGGG----CGGCGaUUCUG- -5' |
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| 17298 | 3' | -57 | NC_004367.1 | + | 106686 | 0.66 | 0.83081 |
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Target: 5'- ---aCCCACCUgccccccagcCCCGUCGCaaccacgaUGAGACa -3' miRNA: 3'- uaagGGGUGGAa---------GGGCGGCG--------AUUCUG- -5' |
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| 17298 | 3' | -57 | NC_004367.1 | + | 122690 | 1.06 | 0.002889 |
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Target: 5'- cAUUCCCCACCUUCCCGCCGCUAAGACc -3' miRNA: 3'- -UAAGGGGUGGAAGGGCGGCGAUUCUG- -5' |
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| 17298 | 3' | -57 | NC_004367.1 | + | 125599 | 1.06 | 0.002889 |
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Target: 5'- cAUUCCCCACCUUCCCGCCGCUAAGACc -3' miRNA: 3'- -UAAGGGGUGGAAGGGCGGCGAUUCUG- -5' |
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| 17298 | 3' | -57 | NC_004367.1 | + | 128508 | 1.06 | 0.002889 |
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Target: 5'- cAUUCCCCACCUUCCCGCCGCUAAGACc -3' miRNA: 3'- -UAAGGGGUGGAAGGGCGGCGAUUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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