Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17306 | 3' | -61.8 | NC_004367.1 | + | 116639 | 0.73 | 0.261927 |
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Target: 5'- uCCAGaACCGCCCagcuccUGGAGCUCCCAGc -3' miRNA: 3'- uGGUCcUGGUGGG------ACCUCGGGGGUUc -5' |
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| 17306 | 3' | -61.8 | NC_004367.1 | + | 56943 | 0.73 | 0.261927 |
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Target: 5'- uCCAGaACCGCCCagcuccUGGAGCUCCCAGc -3' miRNA: 3'- uGGUCcUGGUGGG------ACCUCGGGGGUUc -5' |
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| 17306 | 3' | -61.8 | NC_004367.1 | + | 23582 | 0.73 | 0.250059 |
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Target: 5'- cCCAGaACCGCCCagcuccUGGAGCUCCCAGc -3' miRNA: 3'- uGGUCcUGGUGGG------ACCUCGGGGGUUc -5' |
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| 17306 | 3' | -61.8 | NC_004367.1 | + | 129397 | 1.07 | 0.001081 |
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Target: 5'- cACCAGGACCACCCUGGAGCCCCCAAGu -3' miRNA: 3'- -UGGUCCUGGUGGGACCUCGGGGGUUC- -5' |
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| 17306 | 3' | -61.8 | NC_004367.1 | + | 126488 | 1.07 | 0.001081 |
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Target: 5'- cACCAGGACCACCCUGGAGCCCCCAAGu -3' miRNA: 3'- -UGGUCCUGGUGGGACCUCGGGGGUUC- -5' |
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| 17306 | 3' | -61.8 | NC_004367.1 | + | 120670 | 1.07 | 0.001081 |
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Target: 5'- cACCAGGACCACCCUGGAGCCCCCAAGu -3' miRNA: 3'- -UGGUCCUGGUGGGACCUCGGGGGUUC- -5' |
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| 17306 | 3' | -61.8 | NC_004367.1 | + | 123579 | 1.07 | 0.001081 |
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Target: 5'- cACCAGGACCACCCUGGAGCCCCCAAGu -3' miRNA: 3'- -UGGUCCUGGUGGGACCUCGGGGGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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