Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17318 | 3' | -51 | NC_004367.1 | + | 40805 | 0.66 | 0.981859 |
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Target: 5'- -aGGccAGCACUGuCGCGGCAU-GUUCAa -3' miRNA: 3'- uaCC--UUGUGAC-GCGUCGUAaUAGGUg -5' |
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| 17318 | 3' | -51 | NC_004367.1 | + | 65000 | 0.66 | 0.979612 |
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Target: 5'- -gGGAuagaaGCGCUGCaGCGGCGUgauuugUCACg -3' miRNA: 3'- uaCCU-----UGUGACG-CGUCGUAaua---GGUG- -5' |
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| 17318 | 3' | -51 | NC_004367.1 | + | 47135 | 0.67 | 0.974508 |
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Target: 5'- uUGGuugcaaAACGCaUGCGUgaAGCAUggaUGUCCACg -3' miRNA: 3'- uACC------UUGUG-ACGCG--UCGUA---AUAGGUG- -5' |
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| 17318 | 3' | -51 | NC_004367.1 | + | 104766 | 0.67 | 0.965197 |
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Target: 5'- uUGGAccacauAUGCUGUGCAGUGcu-UCCACa -3' miRNA: 3'- uACCU------UGUGACGCGUCGUaauAGGUG- -5' |
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| 17318 | 3' | -51 | NC_004367.1 | + | 136238 | 0.68 | 0.953725 |
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Target: 5'- -gGGAGCAaa-UGCAGCAUUAgucacugacUCCACa -3' miRNA: 3'- uaCCUUGUgacGCGUCGUAAU---------AGGUG- -5' |
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| 17318 | 3' | -51 | NC_004367.1 | + | 109009 | 0.69 | 0.923753 |
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Target: 5'- gGUGGAGCGC-GCGCAGaCAg---CCAa -3' miRNA: 3'- -UACCUUGUGaCGCGUC-GUaauaGGUg -5' |
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| 17318 | 3' | -51 | NC_004367.1 | + | 134646 | 0.69 | 0.923753 |
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Target: 5'- -cGGAGCAaaGCGCGGCuacAUUAUUgGCu -3' miRNA: 3'- uaCCUUGUgaCGCGUCG---UAAUAGgUG- -5' |
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| 17318 | 3' | -51 | NC_004367.1 | + | 104586 | 0.73 | 0.739035 |
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Target: 5'- uGUGGaAGCACUGCaCAGCAUaugugGUCCAa -3' miRNA: 3'- -UACC-UUGUGACGcGUCGUAa----UAGGUg -5' |
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| 17318 | 3' | -51 | NC_004367.1 | + | 134687 | 0.75 | 0.642859 |
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Target: 5'- cGUGGAuaAUGCUGCGCAGUGU--UCCAUg -3' miRNA: 3'- -UACCU--UGUGACGCGUCGUAauAGGUG- -5' |
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| 17318 | 3' | -51 | NC_004367.1 | + | 134862 | 1.09 | 0.006363 |
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Target: 5'- cAUGGAACACUGCGCAGCAUUAUCCACg -3' miRNA: 3'- -UACCUUGUGACGCGUCGUAAUAGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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