Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17318 | 5' | -48.7 | NC_004367.1 | + | 87392 | 0.66 | 0.998176 |
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Target: 5'- gAGGCCuucuGUGUAGUCGCuCUcugGCg -3' miRNA: 3'- -UUCGGuuauUACAUCGGCGcGAaa-CG- -5' |
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| 17318 | 5' | -48.7 | NC_004367.1 | + | 56847 | 0.66 | 0.996862 |
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Target: 5'- aGGGCCGAgcgGUcUAGCCGC-CUggGCg -3' miRNA: 3'- -UUCGGUUau-UAcAUCGGCGcGAaaCG- -5' |
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| 17318 | 5' | -48.7 | NC_004367.1 | + | 116542 | 0.66 | 0.996862 |
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Target: 5'- aGGGCCGAgcgGUcUAGCCGC-CUggGCg -3' miRNA: 3'- -UUCGGUUau-UAcAUCGGCGcGAaaCG- -5' |
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| 17318 | 5' | -48.7 | NC_004367.1 | + | 13255 | 0.67 | 0.99398 |
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Target: 5'- gAAGCCAAU-GUGgacgccAGCCGuCGgUUUGUa -3' miRNA: 3'- -UUCGGUUAuUACa-----UCGGC-GCgAAACG- -5' |
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| 17318 | 5' | -48.7 | NC_004367.1 | + | 53365 | 0.67 | 0.99398 |
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Target: 5'- uGAGUCAguuuguucgGUGGUGUaaaucuuuuaAGaaGCGCUUUGCg -3' miRNA: 3'- -UUCGGU---------UAUUACA----------UCggCGCGAAACG- -5' |
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| 17318 | 5' | -48.7 | NC_004367.1 | + | 90600 | 0.69 | 0.977364 |
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Target: 5'- gGAGCCAu--AUGUGGCCccguuuggggacGCGCUUUc- -3' miRNA: 3'- -UUCGGUuauUACAUCGG------------CGCGAAAcg -5' |
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| 17318 | 5' | -48.7 | NC_004367.1 | + | 26517 | 0.7 | 0.965267 |
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Target: 5'- uGGCCAGUugugAGUGUuGCCGaCGCcgggUGCg -3' miRNA: 3'- uUCGGUUA----UUACAuCGGC-GCGaa--ACG- -5' |
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| 17318 | 5' | -48.7 | NC_004367.1 | + | 106713 | 0.77 | 0.713272 |
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Target: 5'- -uGCCGGUGGUGUAGUgGgGUUUUGUa -3' miRNA: 3'- uuCGGUUAUUACAUCGgCgCGAAACG- -5' |
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| 17318 | 5' | -48.7 | NC_004367.1 | + | 134824 | 1.11 | 0.008834 |
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Target: 5'- gAAGCCAAUAAUGUAGCCGCGCUUUGCu -3' miRNA: 3'- -UUCGGUUAUUACAUCGGCGCGAAACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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