Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17346 | 3' | -56 | NC_004367.1 | + | 133651 | 0.66 | 0.884725 |
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Target: 5'- uGGCAACauUCCUUGGCC-GCuuucUGC-CAAc -3' miRNA: 3'- -CCGUUG--AGGAACCGGuCGu---GCGaGUU- -5' |
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| 17346 | 3' | -56 | NC_004367.1 | + | 101688 | 0.66 | 0.86248 |
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Target: 5'- uGGCcGCUgCC-UGGCCAGUugGUUg-- -3' miRNA: 3'- -CCGuUGA-GGaACCGGUCGugCGAguu -5' |
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| 17346 | 3' | -56 | NC_004367.1 | + | 22357 | 0.66 | 0.854624 |
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Target: 5'- aGGU--CUCUUUGGCCAGCuAUGCg--- -3' miRNA: 3'- -CCGuuGAGGAACCGGUCG-UGCGaguu -5' |
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| 17346 | 3' | -56 | NC_004367.1 | + | 55949 | 0.66 | 0.854624 |
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Target: 5'- aGGU--CUCUUUGGCCAGCuAUGCg--- -3' miRNA: 3'- -CCGuuGAGGAACCGGUCG-UGCGaguu -5' |
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| 17346 | 3' | -56 | NC_004367.1 | + | 114219 | 0.67 | 0.821175 |
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Target: 5'- uGUGAUUUUUUGGCUucuaGGCACGUUCAGc -3' miRNA: 3'- cCGUUGAGGAACCGG----UCGUGCGAGUU- -5' |
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| 17346 | 3' | -56 | NC_004367.1 | + | 133206 | 0.67 | 0.821175 |
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Target: 5'- gGGCAACguUUgUUGGCgAGgACGCUUGAg -3' miRNA: 3'- -CCGUUG--AGgAACCGgUCgUGCGAGUU- -5' |
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| 17346 | 3' | -56 | NC_004367.1 | + | 74346 | 0.68 | 0.765819 |
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Target: 5'- -cCAACUCCcugagGGCacaaaCGGCACGCUCGGu -3' miRNA: 3'- ccGUUGAGGaa---CCG-----GUCGUGCGAGUU- -5' |
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| 17346 | 3' | -56 | NC_004367.1 | + | 37960 | 0.7 | 0.643855 |
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Target: 5'- aGGCA--UCCUUGGCCAGauauaucacCGCgGCUCAc -3' miRNA: 3'- -CCGUugAGGAACCGGUC---------GUG-CGAGUu -5' |
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| 17346 | 3' | -56 | NC_004367.1 | + | 1484 | 1.08 | 0.002649 |
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Target: 5'- gGGCAACUCCUUGGCCAGCACGCUCAAu -3' miRNA: 3'- -CCGUUGAGGAACCGGUCGUGCGAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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