Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17348 | 3' | -56.1 | NC_004367.1 | + | 25402 | 0.66 | 0.886491 |
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Target: 5'- cCugGGGGUacugugcuguggcGGuGGAGUUGUGUGCGACu -3' miRNA: 3'- -GugUCCUA-------------CCcUUUCGACGCGCGCUG- -5' |
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| 17348 | 3' | -56.1 | NC_004367.1 | + | 40725 | 0.67 | 0.849808 |
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Target: 5'- aCACuaaGGGAUGuuAuuGCUGCGUGUGAUg -3' miRNA: 3'- -GUG---UCCUACccUuuCGACGCGCGCUG- -5' |
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| 17348 | 3' | -56.1 | NC_004367.1 | + | 136565 | 0.69 | 0.741519 |
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Target: 5'- aCACAGcGUGGGAuaauGCgGCGCGaaugaGACg -3' miRNA: 3'- -GUGUCcUACCCUuu--CGaCGCGCg----CUG- -5' |
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| 17348 | 3' | -56.1 | NC_004367.1 | + | 115534 | 0.7 | 0.670845 |
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Target: 5'- cCGCGGGAcgGGGAcAGUUGUcCGCGAg -3' miRNA: 3'- -GUGUCCUa-CCCUuUCGACGcGCGCUg -5' |
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| 17348 | 3' | -56.1 | NC_004367.1 | + | 115492 | 0.71 | 0.598562 |
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Target: 5'- cCGCAGGAcgGGGAcAGUUGUcCGCGAg -3' miRNA: 3'- -GUGUCCUa-CCCUuUCGACGcGCGCUg -5' |
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| 17348 | 3' | -56.1 | NC_004367.1 | + | 59398 | 0.78 | 0.289058 |
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Target: 5'- aCGCuAGGAUGGGGAcuGCUGCG-GCGAUg -3' miRNA: 3'- -GUG-UCCUACCCUUu-CGACGCgCGCUG- -5' |
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| 17348 | 3' | -56.1 | NC_004367.1 | + | 11171 | 1.08 | 0.002782 |
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Target: 5'- gCACAGGAUGGGAAAGCUGCGCGCGACc -3' miRNA: 3'- -GUGUCCUACCCUUUCGACGCGCGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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