Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
17350 | 5' | -50.7 | NC_004367.1 | + | 32968 | 0.68 | 0.964592 |
Target: 5'- gGACGCAUCUaGcCUACGUUgcaCUGGCc -3' miRNA: 3'- -CUGCGUAGA-CuGAUGCGAacaGACUG- -5' |
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17350 | 5' | -50.7 | NC_004367.1 | + | 53930 | 0.71 | 0.911442 |
Target: 5'- --aGCAUCUGaACUGCGCagaaaUCUGGCa -3' miRNA: 3'- cugCGUAGAC-UGAUGCGaac--AGACUG- -5' |
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17350 | 5' | -50.7 | NC_004367.1 | + | 16232 | 0.72 | 0.861452 |
Target: 5'- -uCGCAUUUGGCcAUGgUUGUCUGAUg -3' miRNA: 3'- cuGCGUAGACUGaUGCgAACAGACUG- -5' |
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17350 | 5' | -50.7 | NC_004367.1 | + | 16093 | 1.11 | 0.006306 |
Target: 5'- gGACGCAUCUGACUACGCUUGUCUGACa -3' miRNA: 3'- -CUGCGUAGACUGAUGCGAACAGACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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