Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
17351 | 3' | -48.3 | NC_004367.1 | + | 98657 | 0.66 | 0.998421 |
Target: 5'- gUGAGGCGAGCCUgguUGUGUUUCUc- -3' miRNA: 3'- -AUUUCGUUCGGGagcGUACAAAGAua -5' |
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17351 | 3' | -48.3 | NC_004367.1 | + | 134645 | 0.66 | 0.998421 |
Target: 5'- cAAAGCAcuacauAGUCUUCGCAUG-UUCa-- -3' miRNA: 3'- aUUUCGU------UCGGGAGCGUACaAAGaua -5' |
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17351 | 3' | -48.3 | NC_004367.1 | + | 85321 | 0.75 | 0.787554 |
Target: 5'- -cAGGCAacaaguAGCCCgUCGCAUGggUCUGUa -3' miRNA: 3'- auUUCGU------UCGGG-AGCGUACaaAGAUA- -5' |
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17351 | 3' | -48.3 | NC_004367.1 | + | 16545 | 1.04 | 0.022531 |
Target: 5'- gUAAAGCAAGCCCUCGCAUGUUUCUAUu -3' miRNA: 3'- -AUUUCGUUCGGGAGCGUACAAAGAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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