miRNA: 3'- -UGC-GCgUACGCGUAU--UAGACG-UUACA- -5'
miRNA: 3'- -UGCGCGUACGCGUAUUaga--CGuUACA- -5'
miRNA: 3'- -UGCGCGUACGCGUAu----------UAGACGUUAca -5'
miRNA: 3'- -UGCGCGUACGCGUAUUAGACGUUACA- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17352 | 5' | -49.8 | NC_004367.1 | + | 26538 | 0.67 | 0.99113 |
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Target: 5'- gACGcCGgGUGCGUAUGcuGUCUGUgGGUGUg -3' miRNA: 3'- -UGC-GCgUACGCGUAU--UAGACG-UUACA- -5' |
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| 17352 | 5' | -49.8 | NC_004367.1 | + | 17982 | 0.72 | 0.894937 |
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Target: 5'- uAUGCGCAUGCGCGUGGaaaggGC-GUGUg -3' miRNA: 3'- -UGCGCGUACGCGUAUUaga--CGuUACA- -5' |
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| 17352 | 5' | -49.8 | NC_004367.1 | + | 43076 | 0.76 | 0.712343 |
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Target: 5'- uACGCGCAUGCGUGUcuuuauguccuAUCUGCAGUc- -3' miRNA: 3'- -UGCGCGUACGCGUAu----------UAGACGUUAca -5' |
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| 17352 | 5' | -49.8 | NC_004367.1 | + | 17819 | 1.08 | 0.009919 |
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Target: 5'- cACGCGCAUGCGCAUAAUCUGCAAUGUg -3' miRNA: 3'- -UGCGCGUACGCGUAUUAGACGUUACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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