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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17357 | 5' | -48.6 | NC_004367.1 | + | 77563 | 0.66 | 0.99835 |
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Target: 5'- -gAGCUCCGCgaauUUCUCGGUagCAGc -3' miRNA: 3'- gaUCGAGGCGggu-AAGAGUUAaaGUC- -5' |
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| 17357 | 5' | -48.6 | NC_004367.1 | + | 23119 | 0.69 | 0.988811 |
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Target: 5'- -aAGCUCCGCCC--UCUgGGUUgUAGu -3' miRNA: 3'- gaUCGAGGCGGGuaAGAgUUAAaGUC- -5' |
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| 17357 | 5' | -48.6 | NC_004367.1 | + | 116176 | 0.69 | 0.988811 |
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Target: 5'- -aAGCUCCGCCC--UCUgGGUUgUAGu -3' miRNA: 3'- gaUCGAGGCGGGuaAGAgUUAAaGUC- -5' |
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| 17357 | 5' | -48.6 | NC_004367.1 | + | 63319 | 0.7 | 0.971166 |
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Target: 5'- -aAGCUCaCGCCCGUUgUUGAUaaUCAGg -3' miRNA: 3'- gaUCGAG-GCGGGUAAgAGUUAa-AGUC- -5' |
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| 17357 | 5' | -48.6 | NC_004367.1 | + | 22980 | 1.09 | 0.013955 |
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Target: 5'- uCUAGCUCCGCCCAUUCUCAAUUUCAGc -3' miRNA: 3'- -GAUCGAGGCGGGUAAGAGUUAAAGUC- -5' |
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| 17357 | 5' | -48.6 | NC_004367.1 | + | 56342 | 1.09 | 0.013955 |
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Target: 5'- uCUAGCUCCGCCCAUUCUCAAUUUCAGc -3' miRNA: 3'- -GAUCGAGGCGGGUAAGAGUUAAAGUC- -5' |
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| 17357 | 5' | -48.6 | NC_004367.1 | + | 116037 | 1.09 | 0.013158 |
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Target: 5'- cCUAGCUCCGCCCAUUCUCAAUUUCAGc -3' miRNA: 3'- -GAUCGAGGCGGGUAAGAGUUAAAGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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