Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17358 | 5' | -50.8 | NC_004367.1 | + | 116191 | 0.66 | 0.993022 |
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Target: 5'- aCCuGUaGGGU-GgGACCUAUAGGUAg- -3' miRNA: 3'- -GGuCA-CCCAgUgUUGGAUGUCCAUaa -5' |
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| 17358 | 5' | -50.8 | NC_004367.1 | + | 23134 | 0.66 | 0.993022 |
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Target: 5'- aCCuGUaGGGU-GgGACCUAUAGGUAg- -3' miRNA: 3'- -GGuCA-CCCAgUgUUGGAUGUCCAUaa -5' |
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| 17358 | 5' | -50.8 | NC_004367.1 | + | 143726 | 0.66 | 0.991955 |
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Target: 5'- aCCGGUGGG-CGCGGgacCCUggaGCGGGg--- -3' miRNA: 3'- -GGUCACCCaGUGUU---GGA---UGUCCauaa -5' |
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| 17358 | 5' | -50.8 | NC_004367.1 | + | 18050 | 0.67 | 0.982591 |
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Target: 5'- aCCAG-GGGUgcggcaaguaACAGCCaACAGGUGg- -3' miRNA: 3'- -GGUCaCCCAg---------UGUUGGaUGUCCAUaa -5' |
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| 17358 | 5' | -50.8 | NC_004367.1 | + | 10538 | 0.68 | 0.966705 |
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Target: 5'- uCCAGgauggauGGUUugGACCUACAGGc--- -3' miRNA: 3'- -GGUCac-----CCAGugUUGGAUGUCCauaa -5' |
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| 17358 | 5' | -50.8 | NC_004367.1 | + | 115932 | 0.81 | 0.410982 |
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Target: 5'- cCCGGUGGGUgG-AACCUAUAGGUAUa -3' miRNA: 3'- -GGUCACCCAgUgUUGGAUGUCCAUAa -5' |
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| 17358 | 5' | -50.8 | NC_004367.1 | + | 22876 | 0.81 | 0.410982 |
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Target: 5'- cCCGGUGGGUgG-AACCUAUAGGUAUa -3' miRNA: 3'- -GGUCACCCAgUgUUGGAUGUCCAUAa -5' |
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| 17358 | 5' | -50.8 | NC_004367.1 | + | 23072 | 1.01 | 0.02691 |
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Target: 5'- cCCuGUGGGUCACAACCUACAGGUAUUa -3' miRNA: 3'- -GGuCACCCAGUGUUGGAUGUCCAUAA- -5' |
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| 17358 | 5' | -50.8 | NC_004367.1 | + | 116129 | 1.01 | 0.02691 |
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Target: 5'- cCCuGUGGGUCACAACCUACAGGUAUUa -3' miRNA: 3'- -GGuCACCCAGUGUUGGAUGUCCAUAA- -5' |
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| 17358 | 5' | -50.8 | NC_004367.1 | + | 56434 | 1.09 | 0.00874 |
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Target: 5'- cCCAGUGGGUCACAACCUACAGGUAUUa -3' miRNA: 3'- -GGUCACCCAGUGUUGGAUGUCCAUAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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