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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17365 | 3' | -59.2 | NC_004367.1 | + | 70143 | 0.66 | 0.788716 |
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Target: 5'- cGGGCaCCaGGagGGAgUGACgGUCCAUCa -3' miRNA: 3'- -UCCG-GG-CCa-CUUgACUGgCAGGUGGa -5' |
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| 17365 | 3' | -59.2 | NC_004367.1 | + | 42101 | 0.66 | 0.765023 |
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Target: 5'- -cGCCCGGcagGGGCUGuguaggcccggcaccGCCGUuuGCCUg -3' miRNA: 3'- ucCGGGCCa--CUUGAC---------------UGGCAggUGGA- -5' |
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| 17365 | 3' | -59.2 | NC_004367.1 | + | 112541 | 0.67 | 0.693914 |
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Target: 5'- cGGGCCUGGcauACUGcgaauaaCGUCCGCCa -3' miRNA: 3'- -UCCGGGCCacuUGACug-----GCAGGUGGa -5' |
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| 17365 | 3' | -59.2 | NC_004367.1 | + | 34375 | 0.68 | 0.683029 |
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Target: 5'- uGGCCgcuucauacacuaUGGUGccGCUGACUGUCCGCg- -3' miRNA: 3'- uCCGG-------------GCCACu-UGACUGGCAGGUGga -5' |
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| 17365 | 3' | -59.2 | NC_004367.1 | + | 82598 | 1.08 | 0.001761 |
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Target: 5'- gAGGCCCGGUGAACUGACCGUCCACCUa -3' miRNA: 3'- -UCCGGGCCACUUGACUGGCAGGUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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