Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17370 | 5' | -49.4 | NC_004367.1 | + | 12538 | 0.66 | 0.99755 |
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Target: 5'- gCGAGCauaaUGGCGUCuaauuGCUGUuuacuAAUGGg -3' miRNA: 3'- -GCUCGag--AUCGCGGu----CGAUAu----UUACC- -5' |
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| 17370 | 5' | -49.4 | NC_004367.1 | + | 22369 | 0.68 | 0.99 |
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Target: 5'- uGAGCguugCUcaGGUcucuuugGCCAGCUAUGcGUGGc -3' miRNA: 3'- gCUCGa---GA--UCG-------CGGUCGAUAUuUACC- -5' |
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| 17370 | 5' | -49.4 | NC_004367.1 | + | 55961 | 0.68 | 0.99 |
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Target: 5'- uGAGCguugCUcaGGUcucuuugGCCAGCUAUGcGUGGc -3' miRNA: 3'- gCUCGa---GA--UCG-------CGGUCGAUAUuUACC- -5' |
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| 17370 | 5' | -49.4 | NC_004367.1 | + | 84977 | 0.69 | 0.979231 |
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Target: 5'- uGAGCUC-AGCGUUGGggGUuAAUGGg -3' miRNA: 3'- gCUCGAGaUCGCGGUCgaUAuUUACC- -5' |
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| 17370 | 5' | -49.4 | NC_004367.1 | + | 110859 | 0.7 | 0.961222 |
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Target: 5'- gGGGCUCUAGCugaaaGCCAGacGUGuGUGGc -3' miRNA: 3'- gCUCGAGAUCG-----CGGUCgaUAUuUACC- -5' |
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| 17370 | 5' | -49.4 | NC_004367.1 | + | 104202 | 1.12 | 0.007988 |
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Target: 5'- cCGAGCUCUAGCGCCAGCUAUAAAUGGu -3' miRNA: 3'- -GCUCGAGAUCGCGGUCGAUAUUUACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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