Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17372 | 3' | -55.9 | NC_004367.1 | + | 32284 | 0.66 | 0.874131 |
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Target: 5'- cCGGGGUgaaauaCUgAGUUaGAAGCCAGGCGc -3' miRNA: 3'- -GCCCCGa-----GA-UCGAcUUUCGGUCUGCa -5' |
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| 17372 | 3' | -55.9 | NC_004367.1 | + | 13792 | 0.67 | 0.866693 |
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Target: 5'- gGGGGCgUCU-GC--GAGGUCAGACGa -3' miRNA: 3'- gCCCCG-AGAuCGacUUUCGGUCUGCa -5' |
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| 17372 | 3' | -55.9 | NC_004367.1 | + | 23385 | 0.67 | 0.82383 |
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Target: 5'- gCGGGGUcCUGGcCUGGgcugggagcuccagGAGCUGGGCGg -3' miRNA: 3'- -GCCCCGaGAUC-GACU--------------UUCGGUCUGCa -5' |
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| 17372 | 3' | -55.9 | NC_004367.1 | + | 56747 | 0.67 | 0.82383 |
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Target: 5'- gCGGGGUcCUGGcCUGGgcugggagcuccagGAGCUGGGCGg -3' miRNA: 3'- -GCCCCGaGAUC-GACU--------------UUCGGUCUGCa -5' |
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| 17372 | 3' | -55.9 | NC_004367.1 | + | 116442 | 0.67 | 0.82383 |
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Target: 5'- gCGGGGUcCUGGcCUGGgcugggagcuccagGAGCUGGGCGg -3' miRNA: 3'- -GCCCCGaGAUC-GACU--------------UUCGGUCUGCa -5' |
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| 17372 | 3' | -55.9 | NC_004367.1 | + | 110857 | 1.08 | 0.002971 |
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Target: 5'- cCGGGGCUCUAGCUGAAAGCCAGACGUg -3' miRNA: 3'- -GCCCCGAGAUCGACUUUCGGUCUGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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