Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17372 | 5' | -50.9 | NC_004367.1 | + | 9141 | 0.66 | 0.994957 |
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Target: 5'- -aGCUUCagGGCCucACUCaUGUCaCCCa -3' miRNA: 3'- agUGAAGa-CCGGu-UGAGcAUAGaGGG- -5' |
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| 17372 | 5' | -50.9 | NC_004367.1 | + | 9494 | 0.66 | 0.993223 |
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Target: 5'- aCAUggC-GGCCAuuuccucguacAC-CGUGUCUCCCc -3' miRNA: 3'- aGUGaaGaCCGGU-----------UGaGCAUAGAGGG- -5' |
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| 17372 | 5' | -50.9 | NC_004367.1 | + | 35849 | 0.66 | 0.993223 |
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Target: 5'- -gGCUUCUGGCCuAgUUGUGcgagagaagCUUCCa -3' miRNA: 3'- agUGAAGACCGGuUgAGCAUa--------GAGGG- -5' |
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| 17372 | 5' | -50.9 | NC_004367.1 | + | 86634 | 0.68 | 0.973747 |
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Target: 5'- aCACUgCUGGCUAuguugcgauGCUgGUAUCUCa- -3' miRNA: 3'- aGUGAaGACCGGU---------UGAgCAUAGAGgg -5' |
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| 17372 | 5' | -50.9 | NC_004367.1 | + | 67554 | 0.69 | 0.961012 |
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Target: 5'- uUCAUaUUCUGGCCugggucuGCgCGUAuucauUCUCCCc -3' miRNA: 3'- -AGUG-AAGACCGGu------UGaGCAU-----AGAGGG- -5' |
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| 17372 | 5' | -50.9 | NC_004367.1 | + | 110891 | 1.12 | 0.005626 |
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Target: 5'- cUCACUUCUGGCCAACUCGUAUCUCCCc -3' miRNA: 3'- -AGUGAAGACCGGUUGAGCAUAGAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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