Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17373 | 5' | -46.9 | NC_004367.1 | + | 40198 | 0.66 | 0.999575 |
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Target: 5'- gAGAGUUGAUUGCCCUg-GAGCAa--- -3' miRNA: 3'- -UUUUAACUGACGGGGgaUUUGUgauu -5' |
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| 17373 | 5' | -46.9 | NC_004367.1 | + | 5165 | 0.66 | 0.999463 |
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Target: 5'- ----aUGGCaGCUCCCacgUAAGCACUGGg -3' miRNA: 3'- uuuuaACUGaCGGGGG---AUUUGUGAUU- -5' |
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| 17373 | 5' | -46.9 | NC_004367.1 | + | 37682 | 0.66 | 0.999159 |
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Target: 5'- --uGUUGAgUUGCCCCaauAACACUAu -3' miRNA: 3'- uuuUAACU-GACGGGGgauUUGUGAUu -5' |
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| 17373 | 5' | -46.9 | NC_004367.1 | + | 4707 | 0.67 | 0.998095 |
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Target: 5'- ----gUGACUGCaCaagaCCUGAugGCUGAa -3' miRNA: 3'- uuuuaACUGACG-Gg---GGAUUugUGAUU- -5' |
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| 17373 | 5' | -46.9 | NC_004367.1 | + | 99176 | 0.69 | 0.994539 |
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Target: 5'- ---uUUG-CcGCCCCCUAAGCACc-- -3' miRNA: 3'- uuuuAACuGaCGGGGGAUUUGUGauu -5' |
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| 17373 | 5' | -46.9 | NC_004367.1 | + | 113775 | 1.04 | 0.031456 |
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Target: 5'- aAAAAUUGACUGCCCCCUAAACACUAAc -3' miRNA: 3'- -UUUUAACUGACGGGGGAUUUGUGAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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