Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17374 | 5' | -59.6 | NC_004367.1 | + | 111079 | 0.69 | 0.55483 |
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Target: 5'- gUCCGUGGCuGUGGGGAgauaCGAGUuGGCc- -3' miRNA: 3'- -AGGCGCUG-UACCCCU----GCUCAcCCGua -5' |
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| 17374 | 5' | -59.6 | NC_004367.1 | + | 106862 | 0.7 | 0.506346 |
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Target: 5'- gUUGCGACGgggcugGGGGGCaGGUGGGUGUu -3' miRNA: 3'- aGGCGCUGUa-----CCCCUGcUCACCCGUA- -5' |
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| 17374 | 5' | -59.6 | NC_004367.1 | + | 18676 | 0.71 | 0.42419 |
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Target: 5'- uUCUGUaaGugAUGGGGACGAGUGGa--- -3' miRNA: 3'- -AGGCG--CugUACCCCUGCUCACCcgua -5' |
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| 17374 | 5' | -59.6 | NC_004367.1 | + | 22455 | 0.8 | 0.131278 |
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Target: 5'- gCCGCGGCAUGGGGcCGAGUaGGCu- -3' miRNA: 3'- aGGCGCUGUACCCCuGCUCAcCCGua -5' |
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| 17374 | 5' | -59.6 | NC_004367.1 | + | 56026 | 0.8 | 0.131278 |
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Target: 5'- gCCGCGGCAUGGGGcCGAGUaGGCu- -3' miRNA: 3'- aGGCGCUGUACCCCuGCUCAcCCGua -5' |
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| 17374 | 5' | -59.6 | NC_004367.1 | + | 115441 | 1.06 | 0.001924 |
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Target: 5'- gUCCGCGACAUGGGGACGAGUGGGCAUg -3' miRNA: 3'- -AGGCGCUGUACCCCUGCUCACCCGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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