Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17377 | 5' | -49.8 | NC_004367.1 | + | 101454 | 0.66 | 0.997156 |
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Target: 5'- -aGCGCC-UGGAGgcACCgaaggCaCAAUCUc -3' miRNA: 3'- gaCGCGGaAUCUCaaUGGa----G-GUUAGG- -5' |
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| 17377 | 5' | -49.8 | NC_004367.1 | + | 27427 | 0.66 | 0.996634 |
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Target: 5'- uUGCGC---GGGGUguauUACCUCCAAgaCCu -3' miRNA: 3'- gACGCGgaaUCUCA----AUGGAGGUUa-GG- -5' |
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| 17377 | 5' | -49.8 | NC_004367.1 | + | 45574 | 0.67 | 0.994581 |
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Target: 5'- uUGUGCCUggccAGGGUcACagcggcaaUUCCAGUCCc -3' miRNA: 3'- gACGCGGAa---UCUCAaUG--------GAGGUUAGG- -5' |
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| 17377 | 5' | -49.8 | NC_004367.1 | + | 101628 | 0.67 | 0.99126 |
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Target: 5'- uUGUGCCUUcGG--UGCCUCCAGgcgcuucuuguaccUCCg -3' miRNA: 3'- gACGCGGAAuCUcaAUGGAGGUU--------------AGG- -5' |
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| 17377 | 5' | -49.8 | NC_004367.1 | + | 118623 | 0.72 | 0.907198 |
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Target: 5'- uCUGCGCCUUc-----ACCUUCAGUCCu -3' miRNA: 3'- -GACGCGGAAucucaaUGGAGGUUAGG- -5' |
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| 17377 | 5' | -49.8 | NC_004367.1 | + | 119101 | 1.14 | 0.005397 |
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Target: 5'- gCUGCGCCUUAGAGUUACCUCCAAUCCg -3' miRNA: 3'- -GACGCGGAAUCUCAAUGGAGGUUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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