Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17378 | 3' | -54.2 | NC_004367.1 | + | 10217 | 0.67 | 0.929574 |
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Target: 5'- uGGUUUCugguuuagGGUUGGGUUGCgaCAccGGCGGu -3' miRNA: 3'- -CCAGAG--------UCAACCCAGCGg-GUu-UCGCC- -5' |
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| 17378 | 3' | -54.2 | NC_004367.1 | + | 42031 | 0.71 | 0.798491 |
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Target: 5'- aGGgagCcCAGUUGGGagGCCCAGGgguuaacggugcuGCGGa -3' miRNA: 3'- -CCa--GaGUCAACCCagCGGGUUU-------------CGCC- -5' |
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| 17378 | 3' | -54.2 | NC_004367.1 | + | 129739 | 0.85 | 0.171205 |
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Target: 5'- gGGUCUCAGUUGGGUCcaaCCCGAAG-GGc -3' miRNA: 3'- -CCAGAGUCAACCCAGc--GGGUUUCgCC- -5' |
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| 17378 | 3' | -54.2 | NC_004367.1 | + | 126829 | 0.85 | 0.171205 |
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Target: 5'- gGGUCUCAGUUGGGUCcaaCCCGAAG-GGc -3' miRNA: 3'- -CCAGAGUCAACCCAGc--GGGUUUCgCC- -5' |
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| 17378 | 3' | -54.2 | NC_004367.1 | + | 123920 | 0.85 | 0.171205 |
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Target: 5'- gGGUCUCAGUUGGGUCcaaCCCGAAG-GGc -3' miRNA: 3'- -CCAGAGUCAACCCAGc--GGGUUUCgCC- -5' |
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| 17378 | 3' | -54.2 | NC_004367.1 | + | 121011 | 0.85 | 0.171205 |
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Target: 5'- gGGUCUCAGUUGGGUCcaaCCCGAAG-GGc -3' miRNA: 3'- -CCAGAGUCAACCCAGc--GGGUUUCgCC- -5' |
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| 17378 | 3' | -54.2 | NC_004367.1 | + | 121150 | 1.12 | 0.003114 |
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Target: 5'- gGGUCUCAGUUGGGUCGCCCAAAGCGGc -3' miRNA: 3'- -CCAGAGUCAACCCAGCGGGUUUCGCC- -5' |
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| 17378 | 3' | -54.2 | NC_004367.1 | + | 124059 | 1.12 | 0.003114 |
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Target: 5'- gGGUCUCAGUUGGGUCGCCCAAAGCGGc -3' miRNA: 3'- -CCAGAGUCAACCCAGCGGGUUUCGCC- -5' |
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| 17378 | 3' | -54.2 | NC_004367.1 | + | 126968 | 1.12 | 0.003114 |
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Target: 5'- gGGUCUCAGUUGGGUCGCCCAAAGCGGc -3' miRNA: 3'- -CCAGAGUCAACCCAGCGGGUUUCGCC- -5' |
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| 17378 | 3' | -54.2 | NC_004367.1 | + | 129877 | 1.12 | 0.003114 |
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Target: 5'- gGGUCUCAGUUGGGUCGCCCAAAGCGGc -3' miRNA: 3'- -CCAGAGUCAACCCAGCGGGUUUCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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