Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 17382 | 3' | -56 | NC_004367.1 | + | 127529 | 1.1 | 0.002264 |
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Target: 5'- cCUGAGGCCCCAGGCUUCUUCCCUUAGg -3' miRNA: 3'- -GACUCCGGGGUCCGAAGAAGGGAAUC- -5' |
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| 17382 | 3' | -56 | NC_004367.1 | + | 127691 | 0.7 | 0.689461 |
|
Target: 5'- cCUGGGGCCUCAGGCccgggaCCCUg-- -3' miRNA: 3'- -GACUCCGGGGUCCGaagaa-GGGAauc -5' |
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| 17382 | 3' | -56 | NC_004367.1 | + | 127828 | 0.71 | 0.596604 |
|
Target: 5'- uCUGAGaCaCCAGGCUUCUcCCCUUAu -3' miRNA: 3'- -GACUCcGgGGUCCGAAGAaGGGAAUc -5' |
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| 17382 | 3' | -56 | NC_004367.1 | + | 130138 | 0.84 | 0.115871 |
|
Target: 5'- uCUGAGaCaCCAGGCUUCUUCCCUUAGg -3' miRNA: 3'- -GACUCcGgGGUCCGAAGAAGGGAAUC- -5' |
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| 17382 | 3' | -56 | NC_004367.1 | + | 130238 | 0.97 | 0.016112 |
|
Target: 5'- cCUGAGaCCCCAGGCUUCUUCCCUUAGg -3' miRNA: 3'- -GACUCcGGGGUCCGAAGAAGGGAAUC- -5' |
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| 17382 | 3' | -56 | NC_004367.1 | + | 130338 | 0.93 | 0.030548 |
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Target: 5'- cCUGAGaCCCUAGGCUUCUUCCCUUAGg -3' miRNA: 3'- -GACUCcGGGGUCCGAAGAAGGGAAUC- -5' |
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| 17382 | 3' | -56 | NC_004367.1 | + | 135822 | 0.71 | 0.627602 |
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Target: 5'- uCUGAGGCCCCAGGggUgaUCUUUgAGg -3' miRNA: 3'- -GACUCCGGGGUCCgaAgaAGGGAaUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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