miRNA: 3'- aGUGGGU-GUCGGucCCAC-UUG-GCGCG- -5'
miRNA: 3'- -AGUGGGU-GUCgGU-CCCaCuuGgCGCG- -5'
miRNA: 3'- aGUGGGUGUCGGuccCACUUgGCGCG- -5'
miRNA: 3'- -AGUGGGu------GUCG-GUC-CCAC-UUGG-----CGCG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 174 | 3' | -58.5 | AC_000007.1 | + | 16297 | 0.66 | 0.426999 |
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Target: 5'- cCGCCCGuCGGCC--GGUGcGACgUGCGCg -3' miRNA: 3'- aGUGGGU-GUCGGucCCAC-UUG-GCGCG- -5' |
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| 174 | 3' | -58.5 | AC_000007.1 | + | 13790 | 0.66 | 0.426999 |
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Target: 5'- cUCGCCCAgCAGgCGcGGGcGggUgGUGCg -3' miRNA: 3'- -AGUGGGU-GUCgGU-CCCaCuuGgCGCG- -5' |
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| 174 | 3' | -58.5 | AC_000007.1 | + | 12048 | 0.66 | 0.436703 |
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Target: 5'- aCGCUCACGGCCuu-GUGGA-UGCGCu -3' miRNA: 3'- aGUGGGUGUCGGuccCACUUgGCGCG- -5' |
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| 174 | 3' | -58.5 | AC_000007.1 | + | 23214 | 0.66 | 0.436703 |
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Target: 5'- cUCGCCUucgaucuCAGCgCAGcGGUGcAGCCacaacGCGCa -3' miRNA: 3'- -AGUGGGu------GUCG-GUC-CCAC-UUGG-----CGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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