Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17403 | 3' | -54.8 | NC_004421.1 | + | 3062 | 0.66 | 0.244703 |
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Target: 5'- aCC-AUCCaCGCCUucuuaucugcuuccaACUCCUUUUCUGUCc -3' miRNA: 3'- -GGuUGGG-GUGGA---------------UGGGGAAAAGGCGG- -5' |
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| 17403 | 3' | -54.8 | NC_004421.1 | + | 6209 | 0.67 | 0.195098 |
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Target: 5'- gCCGGCCCCuccCCUGCUCU---UCCcaagauuGCCg -3' miRNA: 3'- -GGUUGGGGu--GGAUGGGGaaaAGG-------CGG- -5' |
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| 17403 | 3' | -54.8 | NC_004421.1 | + | 7376 | 0.67 | 0.189775 |
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Target: 5'- aCAugCaaGCCUACCCCgg--CCGgCa -3' miRNA: 3'- gGUugGggUGGAUGGGGaaaaGGCgG- -5' |
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| 17403 | 3' | -54.8 | NC_004421.1 | + | 2058 | 0.68 | 0.18401 |
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Target: 5'- gCguAUCCagaCUGCCCCUggaCCGCCa -3' miRNA: 3'- -GguUGGGgugGAUGGGGAaaaGGCGG- -5' |
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| 17403 | 3' | -54.8 | NC_004421.1 | + | 1802 | 0.73 | 0.072509 |
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Target: 5'- ----aCCCACCUACCCCc--UCCGUg -3' miRNA: 3'- gguugGGGUGGAUGGGGaaaAGGCGg -5' |
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| 17403 | 3' | -54.8 | NC_004421.1 | + | 23 | 0.73 | 0.065662 |
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Target: 5'- uUCGGCCCC-CUcACCCuCUUUUCCGgCg -3' miRNA: 3'- -GGUUGGGGuGGaUGGG-GAAAAGGCgG- -5' |
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| 17403 | 3' | -54.8 | NC_004421.1 | + | 4304 | 0.74 | 0.055616 |
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Target: 5'- aCCAGCCCCgaucGCCagUGCCCCgaUUCCGa- -3' miRNA: 3'- -GGUUGGGG----UGG--AUGGGGaaAAGGCgg -5' |
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| 17403 | 3' | -54.8 | NC_004421.1 | + | 1828 | 0.77 | 0.035987 |
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Target: 5'- cCCAACCCCGaCUGCCCUUccgaaccuggUCCGUCa -3' miRNA: 3'- -GGUUGGGGUgGAUGGGGAaa--------AGGCGG- -5' |
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| 17403 | 3' | -54.8 | NC_004421.1 | + | 1963 | 1.14 | 0.000036 |
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Target: 5'- gCCAACCCCACCUACCCCUUUUCCGCCu -3' miRNA: 3'- -GGUUGGGGUGGAUGGGGAAAAGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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