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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17409 | 5' | -55.1 | NC_004421.1 | + | 7408 | 0.69 | 0.111975 |
Target: 5'- aUGCCGGCCGGgguaGGcUugcauguacucgagaGACCACCGagGAGCa -3' miRNA: 3'- -AUGGCCGGUUa---CC-G---------------CUGGUGGU--UUCG- -5' |
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17409 | 5' | -55.1 | NC_004421.1 | + | 7355 | 0.8 | 0.013826 |
Target: 5'- -cCCGGCgaCGAUGGCGugCACUGGAGCu -3' miRNA: 3'- auGGCCG--GUUACCGCugGUGGUUUCG- -5' |
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17409 | 5' | -55.1 | NC_004421.1 | + | 3895 | 1.09 | 0.000054 |
Target: 5'- uUACCGGCCAAUGGCGACCACCAAAGCc -3' miRNA: 3'- -AUGGCCGGUUACCGCUGGUGGUUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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