miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
17436 3' -52.8 NC_004453.1 + 482 1.08 0.000916
Target:  5'- uCUUCCUCAGAACCCAAGAGGACAGCUa -3'
miRNA:   3'- -GAAGGAGUCUUGGGUUCUCCUGUCGA- -5'
17436 3' -52.8 NC_004453.1 + 5430 0.66 0.671946
Target:  5'- -cUCCUUucuGcAGCCgaaaGGGAGGGCGGCUg -3'
miRNA:   3'- gaAGGAGu--C-UUGGg---UUCUCCUGUCGA- -5'
17436 3' -52.8 NC_004453.1 + 11901 0.66 0.695138
Target:  5'- uUUCUggcUAGAugCUGAGAGGAgCGGCa -3'
miRNA:   3'- gAAGGa--GUCUugGGUUCUCCU-GUCGa -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.