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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17440 | 5' | -55.8 | NC_004453.1 | + | 13511 | 0.66 | 0.503279 |
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Target: 5'- gACGCGaCUgaaGCGGuGGCcgaaGCGGUggccgaaacGGUGGCg -3' miRNA: 3'- -UGCGC-GA---UGUUuCCG----UGCCA---------CCACCG- -5' |
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| 17440 | 5' | -55.8 | NC_004453.1 | + | 22155 | 0.66 | 0.503279 |
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Target: 5'- uACGCuCUACAGAGGUcggcgagaguagGCGGUaGaGUaGGCa -3' miRNA: 3'- -UGCGcGAUGUUUCCG------------UGCCA-C-CA-CCG- -5' |
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| 17440 | 5' | -55.8 | NC_004453.1 | + | 33111 | 0.67 | 0.481913 |
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Target: 5'- gUGCGCUGCuac-GCAgUGGgagaugGGUGGCg -3' miRNA: 3'- uGCGCGAUGuuucCGU-GCCa-----CCACCG- -5' |
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| 17440 | 5' | -55.8 | NC_004453.1 | + | 17896 | 0.72 | 0.234588 |
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Target: 5'- uACGCGagggaaUGgAAauGGGCACGuUGGUGGCg -3' miRNA: 3'- -UGCGCg-----AUgUU--UCCGUGCcACCACCG- -5' |
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| 17440 | 5' | -55.8 | NC_004453.1 | + | 7356 | 1.1 | 0.000349 |
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Target: 5'- aACGCGCUACAAAGGCACGGUGGUGGCc -3' miRNA: 3'- -UGCGCGAUGUUUCCGUGCCACCACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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