Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17441 | 3' | -48 | NC_004453.1 | + | 21320 | 0.68 | 0.863786 |
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Target: 5'- cGUGAGCgGCGCGGUGguGuggAAACAu -3' miRNA: 3'- uCACUCG-CGCGCUGCguUuaaUUUGU- -5' |
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| 17441 | 3' | -48 | NC_004453.1 | + | 15270 | 0.69 | 0.815672 |
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Target: 5'- aGGcUGGGUGcCGCGACGCGGAgccaGGGCc -3' miRNA: 3'- -UC-ACUCGC-GCGCUGCGUUUaa--UUUGu -5' |
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| 17441 | 3' | -48 | NC_004453.1 | + | 10564 | 0.69 | 0.798968 |
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Target: 5'- uAGUGGGCGacgugcgcgaggcaGUGGCGCAAAaaGAGCGc -3' miRNA: 3'- -UCACUCGCg-------------CGCUGCGUUUaaUUUGU- -5' |
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| 17441 | 3' | -48 | NC_004453.1 | + | 5572 | 0.7 | 0.772917 |
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Target: 5'- gAGUGAGgGCGCuGGCGUAcAUgccGCAg -3' miRNA: 3'- -UCACUCgCGCG-CUGCGUuUAauuUGU- -5' |
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| 17441 | 3' | -48 | NC_004453.1 | + | 13681 | 0.73 | 0.547613 |
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Target: 5'- cGUGuaccuGGUGCGCGACGCGAGcggAAACc -3' miRNA: 3'- uCAC-----UCGCGCGCUGCGUUUaa-UUUGu -5' |
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| 17441 | 3' | -48 | NC_004453.1 | + | 24681 | 0.75 | 0.467403 |
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Target: 5'- cGGUGAGCGCGgccUGACGCAAuagaauGCGg -3' miRNA: 3'- -UCACUCGCGC---GCUGCGUUuaauu-UGU- -5' |
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| 17441 | 3' | -48 | NC_004453.1 | + | 10110 | 1.1 | 0.00258 |
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Target: 5'- aAGUGAGCGCGCGACGCAAAUUAAACAu -3' miRNA: 3'- -UCACUCGCGCGCUGCGUUUAAUUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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