Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17442 | 5' | -50.9 | NC_004453.1 | + | 9718 | 0.66 | 0.843623 |
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Target: 5'- uCUCugUugaGCAuuUCAGAGCCggaggGGCGUUGg -3' miRNA: 3'- -GGGugA---CGUuuAGUCUUGG-----CCGUAGC- -5' |
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| 17442 | 5' | -50.9 | NC_004453.1 | + | 19714 | 0.66 | 0.834256 |
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Target: 5'- aCCA-UGCAAGUCu--GCCGGCcgGUCa -3' miRNA: 3'- gGGUgACGUUUAGucuUGGCCG--UAGc -5' |
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| 17442 | 5' | -50.9 | NC_004453.1 | + | 21961 | 0.66 | 0.803739 |
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Target: 5'- cCCCACaGacgacaguuuagaCGAAaCAGAugUGGCGUCGa -3' miRNA: 3'- -GGGUGaC-------------GUUUaGUCUugGCCGUAGC- -5' |
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| 17442 | 5' | -50.9 | NC_004453.1 | + | 21776 | 0.67 | 0.773414 |
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Target: 5'- gCCGCUGCGAAUaauuuccACCGGCGg-- -3' miRNA: 3'- gGGUGACGUUUAgucu---UGGCCGUagc -5' |
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| 17442 | 5' | -50.9 | NC_004453.1 | + | 9094 | 0.68 | 0.718054 |
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Target: 5'- gCCCGCUGCGcaacauUUGGAACCgGGCugcCGa -3' miRNA: 3'- -GGGUGACGUuu----AGUCUUGG-CCGua-GC- -5' |
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| 17442 | 5' | -50.9 | NC_004453.1 | + | 24275 | 0.68 | 0.695138 |
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Target: 5'- uUCGCgGCGAuucuUCAGAAgCGGCGUUa -3' miRNA: 3'- gGGUGaCGUUu---AGUCUUgGCCGUAGc -5' |
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| 17442 | 5' | -50.9 | NC_004453.1 | + | 14203 | 0.71 | 0.521289 |
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Target: 5'- -gCGCgGCuuGAGUCAGAauAUCGGCGUCGg -3' miRNA: 3'- ggGUGaCG--UUUAGUCU--UGGCCGUAGC- -5' |
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| 17442 | 5' | -50.9 | NC_004453.1 | + | 10162 | 1.13 | 0.000887 |
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Target: 5'- aCCCACUGCAAAUCAGAACCGGCAUCGa -3' miRNA: 3'- -GGGUGACGUUUAGUCUUGGCCGUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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