Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17446 | 3' | -50.9 | NC_004453.1 | + | 19959 | 0.66 | 0.770794 |
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Target: 5'- uAUCUGGCAuugACggggCCcACAGCGGc -3' miRNA: 3'- -UAGACCGUug-UGaaaaGGcUGUCGCC- -5' |
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| 17446 | 3' | -50.9 | NC_004453.1 | + | 17265 | 0.67 | 0.714687 |
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Target: 5'- --aUGGCGuGCACauugCCGACGGCGa -3' miRNA: 3'- uagACCGU-UGUGaaaaGGCUGUCGCc -5' |
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| 17446 | 3' | -50.9 | NC_004453.1 | + | 15502 | 0.68 | 0.668017 |
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Target: 5'- cGUCUGGagaaGCgg--CCGACGGUGGa -3' miRNA: 3'- -UAGACCguugUGaaaaGGCUGUCGCC- -5' |
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| 17446 | 3' | -50.9 | NC_004453.1 | + | 29966 | 0.69 | 0.620719 |
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Target: 5'- -aCUGGCAaaAUACUUcUUCC-AUAGCGGc -3' miRNA: 3'- uaGACCGU--UGUGAA-AAGGcUGUCGCC- -5' |
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| 17446 | 3' | -50.9 | NC_004453.1 | + | 23358 | 0.7 | 0.550327 |
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Target: 5'- -cCUGGCua-GCgaaaCCGGCAGCGGc -3' miRNA: 3'- uaGACCGuugUGaaaaGGCUGUCGCC- -5' |
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| 17446 | 3' | -50.9 | NC_004453.1 | + | 30860 | 0.73 | 0.409024 |
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Target: 5'- -cCUGGCAGCACUUUUuuGAUGGaCa- -3' miRNA: 3'- uaGACCGUUGUGAAAAggCUGUC-Gcc -5' |
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| 17446 | 3' | -50.9 | NC_004453.1 | + | 19541 | 0.95 | 0.014665 |
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Target: 5'- cAUCUGGCAACA-UUUUCCGACAGCGGc -3' miRNA: 3'- -UAGACCGUUGUgAAAAGGCUGUCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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