Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17447 | 3' | -57.2 | NC_004453.1 | + | 18634 | 0.67 | 0.428879 |
Target: 5'- -aGGagGGU--GGGuUCUGGCaugGGGUCCa -3' miRNA: 3'- cgCCa-CCAuuUCC-AGACCGa--CCCGGG- -5' |
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17447 | 3' | -57.2 | NC_004453.1 | + | 1079 | 0.68 | 0.372652 |
Target: 5'- uCGGUGGUGucuAGGuUCU--UUGGGCCUa -3' miRNA: 3'- cGCCACCAUu--UCC-AGAccGACCCGGG- -5' |
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17447 | 3' | -57.2 | NC_004453.1 | + | 15796 | 0.68 | 0.343092 |
Target: 5'- aCGGUGGgcGguuugagaacGGGUCUGGCcaugggaggggcgGGGCgCg -3' miRNA: 3'- cGCCACCauU----------UCCAGACCGa------------CCCGgG- -5' |
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17447 | 3' | -57.2 | NC_004453.1 | + | 20333 | 1.13 | 0.000169 |
Target: 5'- gGCGGUGGUAAAGGUCUGGCUGGGCCCu -3' miRNA: 3'- -CGCCACCAUUUCCAGACCGACCCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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