Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17447 | 5' | -48.6 | NC_004453.1 | + | 10708 | 0.66 | 0.9219 |
Target: 5'- cAAAGugGCCgGCUGA-GUg-GCCACa -3' miRNA: 3'- -UUUCugUGGgCGAUUgCAaaUGGUGg -5' |
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17447 | 5' | -48.6 | NC_004453.1 | + | 30906 | 0.66 | 0.914545 |
Target: 5'- aAAAGuGCugCCagguucuuacuugGCguGCGUUUGCCACCc -3' miRNA: 3'- -UUUC-UGugGG-------------CGauUGCAAAUGGUGG- -5' |
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17447 | 5' | -48.6 | NC_004453.1 | + | 13597 | 0.66 | 0.908233 |
Target: 5'- --uGACGCgggCCGCcacCGUUUcgGCCACCg -3' miRNA: 3'- uuuCUGUG---GGCGauuGCAAA--UGGUGG- -5' |
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17447 | 5' | -48.6 | NC_004453.1 | + | 20052 | 0.66 | 0.908233 |
Target: 5'- -cAGGCGCCCGUcgagAACGgugGCgaGCCa -3' miRNA: 3'- uuUCUGUGGGCGa---UUGCaaaUGg-UGG- -5' |
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17447 | 5' | -48.6 | NC_004453.1 | + | 1005 | 0.66 | 0.907515 |
Target: 5'- -cGGACugUCgGCuUGACGUUUcaguggauuuucaGCCACCc -3' miRNA: 3'- uuUCUGugGG-CG-AUUGCAAA-------------UGGUGG- -5' |
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17447 | 5' | -48.6 | NC_004453.1 | + | 24567 | 0.68 | 0.799845 |
Target: 5'- gGGAGGCGCCCGCagccacacccauuUGugGUUggaaaguCCACa -3' miRNA: 3'- -UUUCUGUGGGCG-------------AUugCAAau-----GGUGg -5' |
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17447 | 5' | -48.6 | NC_004453.1 | + | 13255 | 0.69 | 0.787077 |
Target: 5'- -cAGACGCCCGUcgacgagucuguccUuACGUUUACaaaaGCCu -3' miRNA: 3'- uuUCUGUGGGCG--------------AuUGCAAAUGg---UGG- -5' |
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17447 | 5' | -48.6 | NC_004453.1 | + | 29357 | 0.69 | 0.768521 |
Target: 5'- -uGGGCACggaaCGCUGGCGcauucaUUGCCACUg -3' miRNA: 3'- uuUCUGUGg---GCGAUUGCa-----AAUGGUGG- -5' |
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17447 | 5' | -48.6 | NC_004453.1 | + | 21245 | 0.71 | 0.663965 |
Target: 5'- --cGugGCuCCGCUcuGAUGUUUccacACCACCg -3' miRNA: 3'- uuuCugUG-GGCGA--UUGCAAA----UGGUGG- -5' |
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17447 | 5' | -48.6 | NC_004453.1 | + | 15485 | 0.72 | 0.615942 |
Target: 5'- -uGGACGCCCguGCUAAUGgcguCCACUa -3' miRNA: 3'- uuUCUGUGGG--CGAUUGCaaauGGUGG- -5' |
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17447 | 5' | -48.6 | NC_004453.1 | + | 22218 | 0.72 | 0.59198 |
Target: 5'- ---uGCugCCGgaAACGUUgGCCACCu -3' miRNA: 3'- uuucUGugGGCgaUUGCAAaUGGUGG- -5' |
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17447 | 5' | -48.6 | NC_004453.1 | + | 20299 | 1.1 | 0.002196 |
Target: 5'- gAAAGACACCCGCUAACGUUUACCACCc -3' miRNA: 3'- -UUUCUGUGGGCGAUUGCAAAUGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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