Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17448 | 3' | -55.7 | NC_004453.1 | + | 16707 | 0.66 | 0.581621 |
Target: 5'- gGAGCCgGCaacccuaccgugGCCCuguacuccGAAACGguGGa -3' miRNA: 3'- gCUCGGgCG------------CGGGuuu-----CUUUGCguCC- -5' |
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17448 | 3' | -55.7 | NC_004453.1 | + | 15778 | 0.66 | 0.581621 |
Target: 5'- aCGGGUCUG-GCCauGGGAGGgGCGGGg -3' miRNA: 3'- -GCUCGGGCgCGGguUUCUUUgCGUCC- -5' |
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17448 | 3' | -55.7 | NC_004453.1 | + | 9194 | 0.66 | 0.570426 |
Target: 5'- cCGAGCUCGCucccacugccGCCCucaucgaaGAAGAGGgGgAGGg -3' miRNA: 3'- -GCUCGGGCG----------CGGG--------UUUCUUUgCgUCC- -5' |
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17448 | 3' | -55.7 | NC_004453.1 | + | 13632 | 0.66 | 0.555953 |
Target: 5'- cCGcGCuCUGCGCCUAcguGGAcgucuucggcgucuGACGCGGGc -3' miRNA: 3'- -GCuCG-GGCGCGGGUu--UCU--------------UUGCGUCC- -5' |
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17448 | 3' | -55.7 | NC_004453.1 | + | 21829 | 0.66 | 0.537192 |
Target: 5'- aCGGGCCCuGCGUUUcuuuGGGCGCGGGc -3' miRNA: 3'- -GCUCGGG-CGCGGGuuucUUUGCGUCC- -5' |
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17448 | 3' | -55.7 | NC_004453.1 | + | 10567 | 0.66 | 0.533904 |
Target: 5'- uGGGCgaCGUGCgCGAGGcaguggcgcaaaaaGAGCGCGGGa -3' miRNA: 3'- gCUCGg-GCGCGgGUUUC--------------UUUGCGUCC- -5' |
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17448 | 3' | -55.7 | NC_004453.1 | + | 21207 | 0.67 | 0.504654 |
Target: 5'- aCGGGCagagaGCGCUCAAGGAGACuCcGGa -3' miRNA: 3'- -GCUCGgg---CGCGGGUUUCUUUGcGuCC- -5' |
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17448 | 3' | -55.7 | NC_004453.1 | + | 19575 | 0.69 | 0.40334 |
Target: 5'- aGGGCCaaugGCGCCCAcGGgcGgGguGGg -3' miRNA: 3'- gCUCGGg---CGCGGGUuUCuuUgCguCC- -5' |
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17448 | 3' | -55.7 | NC_004453.1 | + | 15525 | 0.7 | 0.323942 |
Target: 5'- gGAGCCgCGUccGCCCGAAGAGGacuugGCAGu -3' miRNA: 3'- gCUCGG-GCG--CGGGUUUCUUUg----CGUCc -5' |
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17448 | 3' | -55.7 | NC_004453.1 | + | 13554 | 0.71 | 0.300194 |
Target: 5'- -cGGCCCGCGUcagacgCCGAAGAcguccacguaGGCGCAGa -3' miRNA: 3'- gcUCGGGCGCG------GGUUUCU----------UUGCGUCc -5' |
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17448 | 3' | -55.7 | NC_004453.1 | + | 20057 | 0.71 | 0.277814 |
Target: 5'- -cGGCCCagGCGCCCGucGAGaACGguGGc -3' miRNA: 3'- gcUCGGG--CGCGGGUuuCUU-UGCguCC- -5' |
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17448 | 3' | -55.7 | NC_004453.1 | + | 21893 | 1.12 | 0.00031 |
Target: 5'- aCGAGCCCGCGCCCAAAGAAACGCAGGg -3' miRNA: 3'- -GCUCGGGCGCGGGUUUCUUUGCGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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