Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17450 | 5' | -51.4 | NC_004453.1 | + | 26250 | 0.66 | 0.774694 |
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Target: 5'- uUGCGcuGuUUGAGCAGCuCGCC-GGcGCg -3' miRNA: 3'- -ACGCuuC-AACUUGUCG-GUGGuCCuUG- -5' |
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| 17450 | 5' | -51.4 | NC_004453.1 | + | 10981 | 0.67 | 0.695441 |
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Target: 5'- cGCGcuGUcagUGAACggAGCCACUaacaggAGGAGCa -3' miRNA: 3'- aCGCuuCA---ACUUG--UCGGUGG------UCCUUG- -5' |
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| 17450 | 5' | -51.4 | NC_004453.1 | + | 22542 | 0.67 | 0.676659 |
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Target: 5'- gGCGAAGUgggugaggGAcucaggcgcuuaaaaACAGCUACCcGGGGAUu -3' miRNA: 3'- aCGCUUCAa-------CU---------------UGUCGGUGG-UCCUUG- -5' |
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| 17450 | 5' | -51.4 | NC_004453.1 | + | 19185 | 0.69 | 0.601993 |
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Target: 5'- -aUGggGUUGugguccaugggggucGACggccaacgcacaaaGGCCGCCAGGAACa -3' miRNA: 3'- acGCuuCAAC---------------UUG--------------UCGGUGGUCCUUG- -5' |
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| 17450 | 5' | -51.4 | NC_004453.1 | + | 12588 | 0.74 | 0.309776 |
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Target: 5'- cUGCGgcGUUGAuuuuaccuACAGCCGCCugaauaauuugcuGGGAAUa -3' miRNA: 3'- -ACGCuuCAACU--------UGUCGGUGG-------------UCCUUG- -5' |
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| 17450 | 5' | -51.4 | NC_004453.1 | + | 33144 | 1.11 | 0.000807 |
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Target: 5'- cUGCGAAGUUGAACAGCCACCAGGAACg -3' miRNA: 3'- -ACGCUUCAACUUGUCGGUGGUCCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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