Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17453 | 3' | -57 | NC_004453.1 | + | 20898 | 0.66 | 0.447025 |
Target: 5'- -aGGUGUgCAA--GGUGACGCCGUu- -3' miRNA: 3'- aaCCGCGgGUUuuUCGCUGCGGCGcu -5' |
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17453 | 3' | -57 | NC_004453.1 | + | 16402 | 0.66 | 0.417075 |
Target: 5'- gUGGCGCucuugggggCCAGGGAGUuguACGCCGUGc -3' miRNA: 3'- aACCGCG---------GGUUUUUCGc--UGCGGCGCu -5' |
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17453 | 3' | -57 | NC_004453.1 | + | 13536 | 0.67 | 0.361016 |
Target: 5'- -cGGUGgCCGAAAcgguGGCGGC-CCGCGu -3' miRNA: 3'- aaCCGCgGGUUUU----UCGCUGcGGCGCu -5' |
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17453 | 3' | -57 | NC_004453.1 | + | 19582 | 0.68 | 0.318467 |
Target: 5'- aUGGCGCCCAcgggcGGGGUGGgGuuGCa- -3' miRNA: 3'- aACCGCGGGUu----UUUCGCUgCggCGcu -5' |
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17453 | 3' | -57 | NC_004453.1 | + | 1360 | 0.69 | 0.302535 |
Target: 5'- aUGGCGCaaAAuaauAGCGACGgCGUGGa -3' miRNA: 3'- aACCGCGggUUuu--UCGCUGCgGCGCU- -5' |
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17453 | 3' | -57 | NC_004453.1 | + | 21783 | 0.69 | 0.279799 |
Target: 5'- cUGGgGCCCAAcuGGCGGCgaaaGCUGCc- -3' miRNA: 3'- aACCgCGGGUUuuUCGCUG----CGGCGcu -5' |
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17453 | 3' | -57 | NC_004453.1 | + | 13648 | 0.7 | 0.265409 |
Target: 5'- -cGGCGCCCGGAA--UGAC-CCGCGc -3' miRNA: 3'- aaCCGCGGGUUUUucGCUGcGGCGCu -5' |
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17453 | 3' | -57 | NC_004453.1 | + | 10587 | 0.71 | 0.213795 |
Target: 5'- gUGGCGCaaAAAGAGCGcgggacgaGCGCCGaCGGc -3' miRNA: 3'- aACCGCGggUUUUUCGC--------UGCGGC-GCU- -5' |
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17453 | 3' | -57 | NC_004453.1 | + | 21656 | 0.73 | 0.144158 |
Target: 5'- -cGGCGgCCAucaAAAAGCGGCGCaagGCGGu -3' miRNA: 3'- aaCCGCgGGU---UUUUCGCUGCGg--CGCU- -5' |
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17453 | 3' | -57 | NC_004453.1 | + | 11374 | 0.77 | 0.082629 |
Target: 5'- -cGGCGCCa---GAGCGGCGgCGCGAu -3' miRNA: 3'- aaCCGCGGguuuUUCGCUGCgGCGCU- -5' |
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17453 | 3' | -57 | NC_004453.1 | + | 14349 | 1.07 | 0.000437 |
Target: 5'- gUUGGCGCCCAAAAAGCGACGCCGCGAa -3' miRNA: 3'- -AACCGCGGGUUUUUCGCUGCGGCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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