Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17454 | 3' | -58.2 | NC_004453.1 | + | 15064 | 0.66 | 0.462906 |
Target: 5'- aGUCGGCGccuCGaAACUCugCCgCCGCUg -3' miRNA: 3'- aCAGCUGCcu-GC-UUGGGugGG-GGUGG- -5' |
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17454 | 3' | -58.2 | NC_004453.1 | + | 14121 | 0.66 | 0.452965 |
Target: 5'- -uUCGGCGGACGAAaugaUgGCCgaCGCCg -3' miRNA: 3'- acAGCUGCCUGCUUg---GgUGGggGUGG- -5' |
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17454 | 3' | -58.2 | NC_004453.1 | + | 6907 | 0.66 | 0.452965 |
Target: 5'- cGUCGugGuGgGAGCCCAUCUcguuuuuuCCACUa -3' miRNA: 3'- aCAGCugCcUgCUUGGGUGGG--------GGUGG- -5' |
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17454 | 3' | -58.2 | NC_004453.1 | + | 15586 | 0.66 | 0.451977 |
Target: 5'- gUGgaagUGACGGACGuGCCCAaaauagaaaagcgUCCUCGCg -3' miRNA: 3'- -ACa---GCUGCCUGCuUGGGU-------------GGGGGUGg -5' |
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17454 | 3' | -58.2 | NC_004453.1 | + | 28511 | 0.66 | 0.430551 |
Target: 5'- aGUaGcCGGACuGAagcaaaguguuguaGCCCACUCUCACCa -3' miRNA: 3'- aCAgCuGCCUG-CU--------------UGGGUGGGGGUGG- -5' |
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17454 | 3' | -58.2 | NC_004453.1 | + | 1383 | 0.67 | 0.41441 |
Target: 5'- cGUgGAagGGGCGAGCCCcGCCaagCGCCg -3' miRNA: 3'- aCAgCUg-CCUGCUUGGG-UGGgg-GUGG- -5' |
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17454 | 3' | -58.2 | NC_004453.1 | + | 23920 | 0.67 | 0.40509 |
Target: 5'- -cUCGGaagaGGAgGAGCugCCGCCgCCGCCg -3' miRNA: 3'- acAGCUg---CCUgCUUG--GGUGGgGGUGG- -5' |
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17454 | 3' | -58.2 | NC_004453.1 | + | 17792 | 0.67 | 0.377939 |
Target: 5'- uUGUCGGCGGccaacAUGcucuacCCCAUCgCCGCCa -3' miRNA: 3'- -ACAGCUGCC-----UGCuu----GGGUGGgGGUGG- -5' |
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17454 | 3' | -58.2 | NC_004453.1 | + | 6069 | 0.68 | 0.352039 |
Target: 5'- cGUUGACGGcuuuauacggacACGAGCCCuuUUCCACa -3' miRNA: 3'- aCAGCUGCC------------UGCUUGGGugGGGGUGg -5' |
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17454 | 3' | -58.2 | NC_004453.1 | + | 15582 | 0.7 | 0.248219 |
Target: 5'- aGUCcucuuCGGGCGGACgCGgCUCCACCg -3' miRNA: 3'- aCAGcu---GCCUGCUUGgGUgGGGGUGG- -5' |
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17454 | 3' | -58.2 | NC_004453.1 | + | 15515 | 0.72 | 0.180593 |
Target: 5'- gGcCGACGGugGAG-CCGCgUCCGCCc -3' miRNA: 3'- aCaGCUGCCugCUUgGGUGgGGGUGG- -5' |
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17454 | 3' | -58.2 | NC_004453.1 | + | 1448 | 0.73 | 0.166448 |
Target: 5'- cGUCGGCGcuuGGCgGGGCUCGCCCCUuCCa -3' miRNA: 3'- aCAGCUGC---CUG-CUUGGGUGGGGGuGG- -5' |
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17454 | 3' | -58.2 | NC_004453.1 | + | 9180 | 0.76 | 0.106622 |
Target: 5'- aGUCGggGCGGcgccCGAGCUCGCUCCCACUg -3' miRNA: 3'- aCAGC--UGCCu---GCUUGGGUGGGGGUGG- -5' |
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17454 | 3' | -58.2 | NC_004453.1 | + | 14539 | 1.1 | 0.000258 |
Target: 5'- gUGUCGACGGACGAACCCACCCCCACCc -3' miRNA: 3'- -ACAGCUGCCUGCUUGGGUGGGGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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