miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
17455 3' -51.9 NC_004453.1 + 6929 0.66 0.791388
Target:  5'- cACCAcauUAACGCUCAGU-CGuCGUCgUGGu -3'
miRNA:   3'- -UGGUu--AUUGCGGGUCAuGU-GCAG-GCC- -5'
17455 3' -51.9 NC_004453.1 + 11872 0.66 0.781011
Target:  5'- gGCCA-UGAUGCCaAGU-CGCGUCUGu -3'
miRNA:   3'- -UGGUuAUUGCGGgUCAuGUGCAGGCc -5'
17455 3' -51.9 NC_004453.1 + 19577 0.68 0.635377
Target:  5'- gGCCAAUGGCGCCCAcggGCGgGgUgGGg -3'
miRNA:   3'- -UGGUUAUUGCGGGUca-UGUgCaGgCC- -5'
17455 3' -51.9 NC_004453.1 + 15490 0.7 0.5549
Target:  5'- cGCCGugGACGCCC-GUGCuaaugGCGUCCa- -3'
miRNA:   3'- -UGGUuaUUGCGGGuCAUG-----UGCAGGcc -5'
17455 3' -51.9 NC_004453.1 + 13568 0.71 0.514595
Target:  5'- cGCCGAaGACGUCCAcGUAgGCGcagagcgcgggucauUCCGGg -3'
miRNA:   3'- -UGGUUaUUGCGGGU-CAUgUGC---------------AGGCC- -5'
17455 3' -51.9 NC_004453.1 + 12524 0.73 0.386936
Target:  5'- aGCUAGUGAUGCCCGgcucGUACACGUaCGa -3'
miRNA:   3'- -UGGUUAUUGCGGGU----CAUGUGCAgGCc -5'
17455 3' -51.9 NC_004453.1 + 16367 1.1 0.001163
Target:  5'- cACCAAUAACGCCCAGUACACGUCCGGc -3'
miRNA:   3'- -UGGUUAUUGCGGGUCAUGUGCAGGCC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.