Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17455 | 3' | -51.9 | NC_004453.1 | + | 6929 | 0.66 | 0.791388 |
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Target: 5'- cACCAcauUAACGCUCAGU-CGuCGUCgUGGu -3' miRNA: 3'- -UGGUu--AUUGCGGGUCAuGU-GCAG-GCC- -5' |
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| 17455 | 3' | -51.9 | NC_004453.1 | + | 11872 | 0.66 | 0.781011 |
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Target: 5'- gGCCA-UGAUGCCaAGU-CGCGUCUGu -3' miRNA: 3'- -UGGUuAUUGCGGgUCAuGUGCAGGCc -5' |
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| 17455 | 3' | -51.9 | NC_004453.1 | + | 19577 | 0.68 | 0.635377 |
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Target: 5'- gGCCAAUGGCGCCCAcggGCGgGgUgGGg -3' miRNA: 3'- -UGGUUAUUGCGGGUca-UGUgCaGgCC- -5' |
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| 17455 | 3' | -51.9 | NC_004453.1 | + | 15490 | 0.7 | 0.5549 |
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Target: 5'- cGCCGugGACGCCC-GUGCuaaugGCGUCCa- -3' miRNA: 3'- -UGGUuaUUGCGGGuCAUG-----UGCAGGcc -5' |
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| 17455 | 3' | -51.9 | NC_004453.1 | + | 13568 | 0.71 | 0.514595 |
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Target: 5'- cGCCGAaGACGUCCAcGUAgGCGcagagcgcgggucauUCCGGg -3' miRNA: 3'- -UGGUUaUUGCGGGU-CAUgUGC---------------AGGCC- -5' |
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| 17455 | 3' | -51.9 | NC_004453.1 | + | 12524 | 0.73 | 0.386936 |
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Target: 5'- aGCUAGUGAUGCCCGgcucGUACACGUaCGa -3' miRNA: 3'- -UGGUUAUUGCGGGU----CAUGUGCAgGCc -5' |
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| 17455 | 3' | -51.9 | NC_004453.1 | + | 16367 | 1.1 | 0.001163 |
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Target: 5'- cACCAAUAACGCCCAGUACACGUCCGGc -3' miRNA: 3'- -UGGUUAUUGCGGGUCAUGUGCAGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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