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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17456 | 3' | -51.6 | NC_004453.1 | + | 31052 | 0.67 | 0.747455 |
Target: 5'- cCAGcUUCGAUUUGUac-UCAGCcGGCg -3' miRNA: 3'- aGUC-AAGCUAAGCAgccAGUCGaCCG- -5' |
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17456 | 3' | -51.6 | NC_004453.1 | + | 19581 | 0.7 | 0.552416 |
Target: 5'- cCGGagUCGAccugUUGUCGGcuuacCAGCUGGCc -3' miRNA: 3'- aGUCa-AGCUa---AGCAGCCa----GUCGACCG- -5' |
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17456 | 3' | -51.6 | NC_004453.1 | + | 18020 | 1.12 | 0.000841 |
Target: 5'- uUCAGUUCGAUUCGUCGGUCAGCUGGCc -3' miRNA: 3'- -AGUCAAGCUAAGCAGCCAGUCGACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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