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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17459 | 5' | -51.5 | NC_004453.1 | + | 15568 | 0.66 | 0.799393 |
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Target: 5'- gUCGCUCCguCUGUGcccguGGAAGUgacggacGUGCc -3' miRNA: 3'- -AGCGAGGguGGCGCu----CCUUUAa------UACG- -5' |
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| 17459 | 5' | -51.5 | NC_004453.1 | + | 17908 | 0.7 | 0.527226 |
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Target: 5'- aCGCcgCCCAguuaCGCGAGGGAAUggaaauggGCa -3' miRNA: 3'- aGCGa-GGGUg---GCGCUCCUUUAaua-----CG- -5' |
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| 17459 | 5' | -51.5 | NC_004453.1 | + | 22310 | 0.71 | 0.493891 |
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Target: 5'- aCGCUCCgCuuaCGCGAGGGAG--AUGUc -3' miRNA: 3'- aGCGAGG-Gug-GCGCUCCUUUaaUACG- -5' |
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| 17459 | 5' | -51.5 | NC_004453.1 | + | 9199 | 0.72 | 0.42428 |
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Target: 5'- cUCGCUCCCacuGCCGCccucaucgaagaagaGGGGGAGggcauUGCg -3' miRNA: 3'- -AGCGAGGG---UGGCG---------------CUCCUUUaau--ACG- -5' |
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| 17459 | 5' | -51.5 | NC_004453.1 | + | 19269 | 1.14 | 0.000606 |
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Target: 5'- uUCGCUCCCACCGCGAGGAAAUUAUGCa -3' miRNA: 3'- -AGCGAGGGUGGCGCUCCUUUAAUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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