miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
17460 5' -58.4 NC_004453.1 + 12776 0.66 0.371758
Target:  5'- aGGGGCgCAGCUaccacgucgggcaAGACCc-UACgGCCGa -3'
miRNA:   3'- -UCCCGgGUCGG-------------UCUGGaaAUGgUGGC- -5'
17460 5' -58.4 NC_004453.1 + 18920 0.66 0.38167
Target:  5'- -uGG-CCAGCCAGugCacu-CCGCCGg -3'
miRNA:   3'- ucCCgGGUCGGUCugGaaauGGUGGC- -5'
17460 5' -58.4 NC_004453.1 + 14653 0.67 0.32651
Target:  5'- uGGGGUCCGGCCAgcucgcucaugcccGAcuacgugcugcauCCUUcuaucgugccUACCGCCGg -3'
miRNA:   3'- -UCCCGGGUCGGU--------------CU-------------GGAA----------AUGGUGGC- -5'
17460 5' -58.4 NC_004453.1 + 10796 0.7 0.225894
Target:  5'- cGGGUcgcaaacugugaaccUCAGCCGGGCCUUUGaCACCc -3'
miRNA:   3'- uCCCG---------------GGUCGGUCUGGAAAUgGUGGc -5'
17460 5' -58.4 NC_004453.1 + 20272 1.08 0.000294
Target:  5'- aAGGGCCCAGCCAGACCUUUACCACCGc -3'
miRNA:   3'- -UCCCGGGUCGGUCUGGAAAUGGUGGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.