miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
17461 3' -52.7 NC_004453.1 + 9591 0.66 0.693048
Target:  5'- gACGG-UGGgGCUGaUGCAUCUguGGg -3'
miRNA:   3'- aUGCUaACUgCGGCaACGUAGGguCC- -5'
17461 3' -52.7 NC_004453.1 + 26294 0.68 0.565812
Target:  5'- cUGCGAUggGACGUCGgcGCGUCCgAa- -3'
miRNA:   3'- -AUGCUAa-CUGCGGCaaCGUAGGgUcc -5'
17461 3' -52.7 NC_004453.1 + 24510 0.7 0.466216
Target:  5'- aAUGggUGugGCUGcggGCGccUCCCAGGa -3'
miRNA:   3'- aUGCuaACugCGGCaa-CGU--AGGGUCC- -5'
17461 3' -52.7 NC_004453.1 + 25136 1.08 0.001094
Target:  5'- cUACGAUUGACGCCGUUGCAUCCCAGGc -3'
miRNA:   3'- -AUGCUAACUGCGGCAACGUAGGGUCC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.