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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17461 | 5' | -45.5 | NC_004453.1 | + | 16395 | 0.66 | 0.979365 |
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Target: 5'- -uCUUgGGGgCCAGGGAGuUGUAcGCCg -3' miRNA: 3'- guGAAgUUUgGGUUCUUC-ACAUaCGG- -5' |
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| 17461 | 5' | -45.5 | NC_004453.1 | + | 4417 | 0.67 | 0.97012 |
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Target: 5'- aGC-UCAGAgCCAAGggGc--AUGCCa -3' miRNA: 3'- gUGaAGUUUgGGUUCuuCacaUACGG- -5' |
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| 17461 | 5' | -45.5 | NC_004453.1 | + | 3551 | 0.67 | 0.969768 |
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Target: 5'- gGCUUCAAagggGCCCAuugcuauuaucauGGAcgAGUGUAUGg- -3' miRNA: 3'- gUGAAGUU----UGGGU-------------UCU--UCACAUACgg -5' |
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| 17461 | 5' | -45.5 | NC_004453.1 | + | 2424 | 0.67 | 0.965311 |
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Target: 5'- uCACaUCAAACCgCGgacauuguuuugggAGAAGUGggacUGCCg -3' miRNA: 3'- -GUGaAGUUUGG-GU--------------UCUUCACau--ACGG- -5' |
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| 17461 | 5' | -45.5 | NC_004453.1 | + | 25171 | 1.15 | 0.002486 |
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Target: 5'- uCACUUCAAACCCAAGAAGUGUAUGCCg -3' miRNA: 3'- -GUGAAGUUUGGGUUCUUCACAUACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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