Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
17471 | 3' | -51.9 | NC_004456.1 | + | 34709 | 0.66 | 0.816972 |
Target: 5'- uGAUGGCCAuGacCCGGUacgGcaUGACCGCc -3' miRNA: 3'- -CUACCGGU-CaaGGUCAa--C--AUUGGCGa -5' |
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17471 | 3' | -51.9 | NC_004456.1 | + | 7724 | 0.66 | 0.816972 |
Target: 5'- cGAUGaGCUGGUcgaUuuGGUUGaGGCCGCUg -3' miRNA: 3'- -CUAC-CGGUCA---AggUCAACaUUGGCGA- -5' |
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17471 | 3' | -51.9 | NC_004456.1 | + | 18726 | 0.66 | 0.816972 |
Target: 5'- -cUGGCCAGcauaUCCGGcucaauaaucUGUAGCUGCa -3' miRNA: 3'- cuACCGGUCa---AGGUCa---------ACAUUGGCGa -5' |
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17471 | 3' | -51.9 | NC_004456.1 | + | 16874 | 0.66 | 0.795906 |
Target: 5'- uGGUGGCCgcuggugAGUUCCGGUcGauuaAGCCGUUu -3' miRNA: 3'- -CUACCGG-------UCAAGGUCAaCa---UUGGCGA- -5' |
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17471 | 3' | -51.9 | NC_004456.1 | + | 28889 | 0.66 | 0.77612 |
Target: 5'- --cGaGCCAuuGUUCCAGUUGcccCCGCg -3' miRNA: 3'- cuaC-CGGU--CAAGGUCAACauuGGCGa -5' |
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17471 | 3' | -51.9 | NC_004456.1 | + | 41272 | 0.68 | 0.698767 |
Target: 5'- gGGUGGCCAGgcggacucgCCAGa-GUAACCGa- -3' miRNA: 3'- -CUACCGGUCaa-------GGUCaaCAUUGGCga -5' |
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17471 | 3' | -51.9 | NC_004456.1 | + | 32657 | 0.69 | 0.59237 |
Target: 5'- ---aGCCGGUUCCAGUUGauagaagugcugAGCCGUg -3' miRNA: 3'- cuacCGGUCAAGGUCAACa-----------UUGGCGa -5' |
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17471 | 3' | -51.9 | NC_004456.1 | + | 794 | 0.7 | 0.583143 |
Target: 5'- uGUGGCCAGUUCCuuGaUGUGuUCGCc -3' miRNA: 3'- cUACCGGUCAAGGu-CaACAUuGGCGa -5' |
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17471 | 3' | -51.9 | NC_004456.1 | + | 19872 | 0.7 | 0.571651 |
Target: 5'- --gGGCCAucGUUCCAGUgauugGUGGCCugGCg -3' miRNA: 3'- cuaCCGGU--CAAGGUCAa----CAUUGG--CGa -5' |
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17471 | 3' | -51.9 | NC_004456.1 | + | 7837 | 1.07 | 0.001852 |
Target: 5'- uGAUGGCCAGUUCCAGUUGUAACCGCUu -3' miRNA: 3'- -CUACCGGUCAAGGUCAACAUUGGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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