Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17471 | 5' | -53.4 | NC_004456.1 | + | 37358 | 0.66 | 0.752296 |
Target: 5'- gGCuGCGGCCUCu---GAGUUGGCCu- -3' miRNA: 3'- -UGuCGCCGGAGuuggUUUAGCUGGuc -5' |
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17471 | 5' | -53.4 | NC_004456.1 | + | 30700 | 0.66 | 0.730586 |
Target: 5'- --cGCGGCCacUCAAuCCAAcgUGAUCGGc -3' miRNA: 3'- uguCGCCGG--AGUU-GGUUuaGCUGGUC- -5' |
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17471 | 5' | -53.4 | NC_004456.1 | + | 16175 | 0.66 | 0.719565 |
Target: 5'- uCGGCGGCggCGuCCAGGUCGAacguacacucguCCAGa -3' miRNA: 3'- uGUCGCCGgaGUuGGUUUAGCU------------GGUC- -5' |
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17471 | 5' | -53.4 | NC_004456.1 | + | 32238 | 0.67 | 0.685994 |
Target: 5'- cCAGCuGCaaggCAGCCAAAccaCGGCCAGa -3' miRNA: 3'- uGUCGcCGga--GUUGGUUUa--GCUGGUC- -5' |
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17471 | 5' | -53.4 | NC_004456.1 | + | 34689 | 0.67 | 0.64051 |
Target: 5'- uGCAGCGGCaacgaauaUCAACCccuuGGUCaauCCAGg -3' miRNA: 3'- -UGUCGCCGg-------AGUUGGu---UUAGcu-GGUC- -5' |
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17471 | 5' | -53.4 | NC_004456.1 | + | 10832 | 0.7 | 0.505825 |
Target: 5'- aAUAGCgucacGGCCaauaUCAACCAugauAUCGACCAu -3' miRNA: 3'- -UGUCG-----CCGG----AGUUGGUu---UAGCUGGUc -5' |
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17471 | 5' | -53.4 | NC_004456.1 | + | 15493 | 0.74 | 0.294925 |
Target: 5'- gGCAGCGGCCuucacuUCAucaacuauuuccugaGCCAcAUCGGCCAc -3' miRNA: 3'- -UGUCGCCGG------AGU---------------UGGUuUAGCUGGUc -5' |
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17471 | 5' | -53.4 | NC_004456.1 | + | 14733 | 0.77 | 0.200265 |
Target: 5'- cCAGCGGCC--AACCuuAUCGACCAa -3' miRNA: 3'- uGUCGCCGGagUUGGuuUAGCUGGUc -5' |
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17471 | 5' | -53.4 | NC_004456.1 | + | 28201 | 0.77 | 0.179118 |
Target: 5'- gGCGGCGGCCUCuggugucgcAACUAuggCGACCGGu -3' miRNA: 3'- -UGUCGCCGGAG---------UUGGUuuaGCUGGUC- -5' |
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17471 | 5' | -53.4 | NC_004456.1 | + | 7799 | 1.08 | 0.001331 |
Target: 5'- uACAGCGGCCUCAACCAAAUCGACCAGc -3' miRNA: 3'- -UGUCGCCGGAGUUGGUUUAGCUGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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