Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17472 | 3' | -56.2 | NC_004456.1 | + | 33372 | 0.66 | 0.611469 |
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Target: 5'- -aGCUCCaagaaauGCGCCAGGcacuGAACCgAGcCGGAg -3' miRNA: 3'- agCGAGG-------UGUGGUUC----CUUGG-UC-GCCU- -5' |
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| 17472 | 3' | -56.2 | NC_004456.1 | + | 26359 | 0.66 | 0.590338 |
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Target: 5'- -aGCUCCACAUCAAaguGGAucacACCgcgcuuGGCGGu -3' miRNA: 3'- agCGAGGUGUGGUU---CCU----UGG------UCGCCu -5' |
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| 17472 | 3' | -56.2 | NC_004456.1 | + | 20376 | 0.67 | 0.49296 |
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Target: 5'- gCGCUCaucguCACUGAGGA-CCuuauGCGGAg -3' miRNA: 3'- aGCGAGgu---GUGGUUCCUuGGu---CGCCU- -5' |
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| 17472 | 3' | -56.2 | NC_004456.1 | + | 32474 | 0.68 | 0.462096 |
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Target: 5'- gCGCaaacCUAUGCCGGGGGACCuucGUGGAc -3' miRNA: 3'- aGCGa---GGUGUGGUUCCUUGGu--CGCCU- -5' |
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| 17472 | 3' | -56.2 | NC_004456.1 | + | 37119 | 0.72 | 0.286796 |
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Target: 5'- uUUGCUCCACguaACCuuGGGAAaacggUCAGCGGGa -3' miRNA: 3'- -AGCGAGGUG---UGGu-UCCUU-----GGUCGCCU- -5' |
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| 17472 | 3' | -56.2 | NC_004456.1 | + | 34203 | 0.74 | 0.198295 |
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Target: 5'- gCGCUUagggguaaGCACUAGGGAuGCUAGCGGAa -3' miRNA: 3'- aGCGAGg-------UGUGGUUCCU-UGGUCGCCU- -5' |
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| 17472 | 3' | -56.2 | NC_004456.1 | + | 41265 | 0.75 | 0.182729 |
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Target: 5'- -aGCUCCGCACCGAGuucuGCCAGCa-- -3' miRNA: 3'- agCGAGGUGUGGUUCcu--UGGUCGccu -5' |
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| 17472 | 3' | -56.2 | NC_004456.1 | + | 11998 | 1.09 | 0.000578 |
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Target: 5'- uUCGCUCCACACCAAGGAACCAGCGGAc -3' miRNA: 3'- -AGCGAGGUGUGGUUCCUUGGUCGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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