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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 17472 | 5' | -51.9 | NC_004456.1 | + | 11964 | 1.11 | 0.001039 |
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Target: 5'- cAUCCCACCGACUCCAAGAGAAAAGCCa -3' miRNA: 3'- -UAGGGUGGCUGAGGUUCUCUUUUCGG- -5' |
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| 17472 | 5' | -51.9 | NC_004456.1 | + | 12558 | 0.68 | 0.724026 |
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Target: 5'- -gCCCACCG-CUUCAucgauaaAGcgcGGggGAGCCu -3' miRNA: 3'- uaGGGUGGCuGAGGU-------UC---UCuuUUCGG- -5' |
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| 17472 | 5' | -51.9 | NC_004456.1 | + | 18302 | 0.73 | 0.395691 |
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Target: 5'- cUCCCACCGGCa-CGAGcAGGccaGAGGCCa -3' miRNA: 3'- uAGGGUGGCUGagGUUC-UCU---UUUCGG- -5' |
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| 17472 | 5' | -51.9 | NC_004456.1 | + | 20865 | 0.68 | 0.702671 |
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Target: 5'- cUCCUggGCUuGCUCC-AGAGAAAGGUCc -3' miRNA: 3'- uAGGG--UGGcUGAGGuUCUCUUUUCGG- -5' |
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| 17472 | 5' | -51.9 | NC_004456.1 | + | 28693 | 0.68 | 0.691315 |
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Target: 5'- -cUCUGCCGGCUUCAGGAu---GGCCa -3' miRNA: 3'- uaGGGUGGCUGAGGUUCUcuuuUCGG- -5' |
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| 17472 | 5' | -51.9 | NC_004456.1 | + | 31243 | 0.68 | 0.725141 |
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Target: 5'- uGUUCUGCCGACUCUAAGu---GGGCa -3' miRNA: 3'- -UAGGGUGGCUGAGGUUCucuuUUCGg -5' |
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| 17472 | 5' | -51.9 | NC_004456.1 | + | 31662 | 0.66 | 0.799882 |
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Target: 5'- cUCCaaaACUGACUCuCAAGAccGAGGAGUUc -3' miRNA: 3'- uAGGg--UGGCUGAG-GUUCU--CUUUUCGG- -5' |
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| 17472 | 5' | -51.9 | NC_004456.1 | + | 41258 | 0.66 | 0.789682 |
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Target: 5'- -gCCCACCaGCUCCGcaccGAGuucuGCCa -3' miRNA: 3'- uaGGGUGGcUGAGGUu---CUCuuuuCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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